Simultaneous detection and quantification of amphetamines, diazepam and its metabolites, cocaine and its metabolites, and opiates in hair by LC-ESI-MS-MS using a single extraction method

Journal of Analytical Toxicology
Eleanor I MillerJohn S Oliver

Abstract

A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous identification and quantification of amphetamines, diazepam and its metabolites, cocaine and its metabolites, and opiates from hair using a single extraction method. As part of the method development, Gemini C18, Synergi Hydro RP, and Zorbax Stablebond-Phenyl LC columns were tested with three different mobile phases. Analyte recovery and limit of detection were evaluated for two different solid-phase extraction methods that used Bond Elut Certify and Clean Screen cartridges. Phosphate buffer (pH 5.0) was chosen as the optimum hair incubation medium because of the high stability of cocaine and 6-monoacetylmorphine using this method and faster sample preparation. The optimized method was fully validated. Linearity was established over the concentration range 0.2-10 ng/mg hair, and the correlation coefficients were all greater than 0.99. Total extraction recoveries were greater than 76%, detection limits were between 0.02 and 0.09 ng/mg, and the intra- and interday imprecisions were generally less than 20% in spiked hair. The intra- and interbatch imprecision of the method for a pooled authentic hair sample ranged from 1.4 to 2...Continue Reading

Citations

Mar 17, 2010·Analytical and Bioanalytical Chemistry·Mitsuhiro WadaKenichiro Nakashima
Feb 23, 2011·Analytical and Bioanalytical Chemistry·Susanna VogliardiSanto Davide Ferrara
Jul 1, 2009·Bioanalysis·Victoria F SamanidouIoannis N Papadoyannis
Sep 9, 2011·Bioanalysis·Victoria SamanidouKonstantinos Rentifis
Apr 20, 2010·Analytical and Bioanalytical Chemistry·P LópezM Chiarotti
May 25, 2013·Analytical and Bioanalytical Chemistry·Marcela Nogueira Rabelo AlvesAldo Polettini
Dec 3, 2011·Forensic Science International : Synergy·Elena LendoiroMarta Concheiro
Mar 15, 2011·Biomedical Chromatography : BMC·Mitsuhiro Nakamura
Nov 21, 2012·Mass Spectrometry Reviews·Marco VincentiValentina Pirro
Mar 31, 2015·Analytica Chimica Acta·Anneli KruveIvo Leito
Jul 15, 2015·Bioanalysis·Mário Barroso, Eugenia Gallardo
Apr 30, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ji Yeon ChoiSun Young Baek
Oct 8, 2013·Journal of Pharmaceutical and Biomedical Analysis·Camilla MontesanoMarie Katrine Klose Nielsen
Apr 30, 2014·International Journal of Legal Medicine·Julie MaublancJean-Michel Gaulier
Jan 22, 2015·Analytica Chimica Acta·Susanna VogliardiDonata Favretto
Feb 17, 2009·Journal of Separation Science·Steen Honoré Hansen
May 9, 2009·Analytical Chemistry·T A BrettellJ R Almirall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Francesca BucelliNicola Comodo
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Rosa Cordero, Sue Paterson
Journal of Analytical Toxicology
S HegstadAsbjorg Solberg Christophersen
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Małgorzata KłysMariusz Scisłowski
© 2022 Meta ULC. All rights reserved