Simultaneous detection of bovine arboviruses using single-tube multiplex reverse transcription-polymerase chain reaction

Journal of Virological Methods
S OhashiT Tsuda

Abstract

Single-tube multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed to detect and identify arboviruses in infected cell-culture fluids and field specimens. The technique was equally sensitive for detecting five different viruses in cell cultures, namely the Chuzan, Ibaraki, and Bluetongue viruses belonging to Orbivirus, and the Akabane virus and Peaton virus belonging to Orthobunyavirus, and was less sensitive than former viruses for detecting Aino virus belonging to Orthobunyavirus. The mRT-PCR reliably detected 0.6-10(3.1) median tissue culture infective doses. The mRT-PCR readily identified viruses by discriminating the size of their amplified gene products. The technique was as sensitive as virus isolation in detecting single infected plasma in five plasmas from sentinel cattle and in detecting two infectious homogenates in eight homogenates of Culicoides biting midges. The mRT-PCR may be a sensitive and rapid assay for surveillance of bovine arboviruses in field specimens.

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Citations

Jan 6, 2012·Journal of Biomedicine & Biotechnology·Nicholas JohnsonAnthony R Fooks
Mar 6, 2010·Emerging Infectious Diseases·Belen Rodriguez-SanchezJose M Sanchez-Vizcaino
Mar 18, 2011·Biologicals : Journal of the International Association of Biological Standardization·Yeon-Hee KimSe Chang Park
Jun 9, 2009·Journal of Virological Methods·Imadeldin E AradaibMohamed A Abdalla
Mar 10, 2015·Journal of Virological Methods·Kerstin WernikeMartin Beer
Apr 24, 2012·Journal of Comparative Pathology·J K OemI S Roh
Mar 18, 2017·The Journal of Veterinary Medical Science·Suryo Purnomo EdiHiroaki Shirafuji
Sep 8, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·H Jane Oakey
Nov 13, 2018·Journal of Medical Entomology·Tohru YanaseShogo Tanaka
Jun 6, 2007·Pest Management Science·James G Logan, Michael A Birkett
Oct 9, 2008·The Journal of Veterinary Medical Science·Dong-Kun YangKyoung-Oh Cho
May 29, 2015·The Journal of Veterinary Medical Science·Yoshimasa HirashimaTohru Yanase
Sep 7, 2017·Virology Journal·Akifumi OhtaniYasuo Inoshima
Apr 18, 2018·Emerging Infectious Diseases·Yuka KamomaeHiroaki Shirafuji
Sep 1, 2021·Vector Borne and Zoonotic Diseases·Katsunori MurotaTohru Yanase

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