Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells.

Angewandte Chemie
Jonathan A RobsonJames D E T Wilton-Ely

Abstract

A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.

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Citations

Dec 24, 2020·Angewandte Chemie·Fengdong QuMinghui Yang
Feb 19, 2021·Chemical Communications : Chem Comm·Jinye NiuBo Tang
Dec 20, 2020·Chemistry : a European Journal·Haibin XiaoBo Tang
Feb 4, 2021·Angewandte Chemie·Francesca PiazzollaStefan Matile
Apr 20, 2021·Chemical Communications : Chem Comm·José García-CalvoStefan Matile
Aug 20, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Chen GengWeiying Lin

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Methods Mentioned

BETA
NMR
fluorescence spectroscopy

Software Mentioned

ImageJ

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