PMID: 12781944Jun 5, 2003Paper

Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR

International Journal of Food Microbiology
Arvind A Bhagwat

Abstract

A protocol enabling simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains was devised and evaluated using artificially contaminated fresh produce. Association of Official Analytical Chemists (AOAC)-approved polymerase chain reaction (PCR) detection methods for three human pathogens were modified to enable simultaneous and real-time detection with high throughput capability. The method includes a melting-curve analysis of PCR products, which serves as confirmatory test. The modified protocol successfully detected all three pathogens when fresh produce was washed with artificially contaminated water containing E. coli O157:H7 and S. typhimurium down to the predicted level of 1 to 10 cells/ml and L. monocytogenes at 1000 cells/ml. The ability to monitor several pathogens simultaneously will save time and increase our ability to assure food safety.

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Citations

Aug 4, 2009·Applied Biochemistry and Biotechnology·Ghinwa NajaJohn H T Luong
Jan 9, 2004·Applied and Environmental Microbiology·Chris Rowe TaittFrances S Ligler
Oct 7, 2004·Applied and Environmental Microbiology·David Rodríguez-LázaroMaria Pla
Apr 22, 2011·The Journal of Parasitology·Laura F Lalonde, Alvin A Gajadhar
Jun 26, 2014·TheScientificWorldJournal·Pierangeli G VitalWindell L Rivera
Jun 7, 2014·Applied Microbiology and Biotechnology·Min Seok ChoDong Suk Park
Oct 19, 2010·Sensing and Instrumentation for Food Quality and Safety·Jason S KimGeorge P Anderson
Jan 25, 2011·Journal of Microbiological Methods·Urban TraunšekMinka Kovač
Jul 9, 2004·International Journal of Food Microbiology·Rolf Reissbrodt
Sep 12, 2006·Journal of Microbiological Methods·Alka Mukhopadhyay, Utpal K Mukhopadhyay
Nov 29, 2005·Journal of Microbiological Methods·Robin L T ChurchillJ Christopher Hall
Jan 21, 2006·Systematic and Applied Microbiology·Michael KlockeSusanne Barth
Jan 16, 2015·Biosensors & Bioelectronics·Sang-Hee LeeYang-Hoon Kim
Oct 13, 2005·FEMS Microbiology Reviews·Uta GasanovPhilip M Hansbro
Jan 20, 2009·Acta Veterinaria Hungarica·Jitu R Patel, Arvind A Bhagwat
Jan 21, 2012·Journal of Microbiological Methods·Snehal JadhavEnzo A Palombo
Jul 28, 2016·International Journal of Food Microbiology·Abigail B SnyderAhmed E Yousef
Jul 30, 2004·International Journal of Food Microbiology·Samantha Hu Liming, Arvind A Bhagwat
Nov 14, 2016·Journal of Dairy Science·José M LandeteJuan L Arqués
Mar 4, 2011·Journal of Environmental Monitoring : JEM·Gha-Young KimAhjeong Son

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