PMID: 7713941Apr 7, 1995

Simultaneous detection of free radical release and membrane current during phagocytosis

The Journal of Biological Chemistry
K O Holevinsky, D J Nelson

Abstract

Stimulation of macrophages induces the "respiratory burst" response which is associated with the generation of superoxide (O2-), a drop in cytoplasmic pH, and a pronounced depolarization of the membrane potential. The purpose of the present studies was to determine whether an increase in O2- was temporally related to changes in membrane potential and transmembrane current. Release of O2- at the single cell level was photometrically monitored during phagocytosis of immune complexes while simultaneously measuring whole-cell current. Membrane depolarization and the generation of a non-selective current followed an increase in O2- production with a variable lag time which was correlated with the state of cellular maturation in culture. In the absence of phagocytosis, the exposure of macrophages to O2- generated by a xanthine-xanthine oxidase reaction activated a non-selective current similar to that seen after phagocytosis. These results provide the first demonstration of the relationship between free radical release and the ensuing electrophysiological signaling events which are linked to particle engulfment in phagocytic cells.

References

Jan 10, 2009·European Biophysics Journal : EBJ·Darío Manuel DomínguezMarcela Camacho
Jan 22, 1998·Free Radical Biology & Medicine·I A GamaleyI V Klyubin
Dec 10, 2002·International Immunopharmacology·C JacobV Lagente
Feb 2, 2010·Nature Immunology·Tiffany M LinkMichael J Caterina
Apr 22, 2008·Photochemistry and Photobiology·Nasser A DaghastanliMauricio S Baptista
Dec 2, 2004·Molecular and Cellular Biology·Regina A ClemensErik J Peterson
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q H LiuB D Freedman
Dec 15, 2010·Experimental Neurology·Wendy W YangLamya S Shihabuddin
Oct 21, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Amir J GuriJosep Bassaganya-Riera
Sep 6, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Andrew R Bartholomaeus, Victoria S Haritos
Nov 19, 2011·FEMS Immunology and Medical Microbiology·Lisette M CarrithersMichael D Carrithers
Apr 9, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R M SaraivaG M Oliveira-Castro
Sep 7, 2012·Multiple Sclerosis : Clinical and Laboratory Research·Joel A BlackStephen G Waxman
Aug 9, 2007·Journal of Leukocyte Biology·Elizabeth F Vernon-WilsonSimon B Brown
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brice CampoR Alan North
Aug 12, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amanda B MackenzieR Alan North
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael D CarrithersStephen G Waxman

Citations

Aug 1, 1979·The Journal of Cell Biology·P E NewburgerH J Cohen
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H M Korchak, G Weissmann
Apr 1, 1975·The Journal of Infectious Diseases·L R DeChateletC E McCall
Jan 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J StuehrC F Nathan
Jul 1, 1990·The Journal of Membrane Biology·D J NelsonF Jow
Jan 1, 1989·Methods in Cell Biology·R Y Tsien
Jan 1, 1981·Journal of Cellular Physiology·G S JonesV Castranova
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
May 1, 1983·Journal of Cellular Physiology·B E Seligmann, J I Gallin
Jul 1, 1993·Pflügers Archiv : European journal of physiology·M OikeH Kuriyama
Oct 22, 1993·Proceedings. Biological Sciences·G CharpentierG Brûlé
Feb 1, 1993·Trends in Biochemical Sciences·A W Segal, A Abo

Related Concepts

Xanthine
Resting Potentials
Free Radicals
Respiratory Burst
Fc Receptor
Biologic Development
Superoxides
Xanthine Oxidase
Integral to Membrane
Cytoplasmic

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