Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Keiko NarumiToshiro Motoya

Abstract

We developed an analytical method for the simultaneous determination of tea catechins and gallic acid (GA) in human serum using ion-pair high-performance liquid chromatography (HPLC) with electrochemical detection. GA was measured to estimate the amount of gallate moiety produced by degradation of gallated catechins ((-)-epicatechin-3-gallate, ECG; (-)-epigallocatechin-3-gallate, EGCG). Ethyl gallate was adopted as an internal standard to correct for the extraction efficiency. To maximize extraction efficiency, a hydrophobic polytetrafluoroethylene (PTFE) filter was selected for pre-treatment prior to separation. HPLC separation was performed using a C18 reversed-phase column with a gradient mobile phase of phosphate buffer (pH 2.5) containing tetrahexylammonium hydrogensulfate as an ion-pair reagent. Using this method, (-)-epicatechin (EC), (-)-epigallocatechin (EGC), ECG, EGCG, ethyl gallate, and GA were detected as single peaks. The resolution values for target analytes were 4.0-13.0 and the mean values of the absolute recoveries of catechins and GA were 77.3-93.9%. The detection limits for catechins and GA in serum were 0.4-3.1ng/mL. The serum catechin levels of eight healthy volunteers after ingestion of a single dose of g...Continue Reading

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Citations

Dec 20, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin DongHua Yang
Mar 29, 2014·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·I OnakpoyaM Thompson
Sep 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhuo-Yu CaiYue-Rong Liang
Feb 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamed-Yassine AmarouchHugues Abriel
Oct 1, 2020·Antioxidants·Josipa GrgićAna Bucić-Kojić
May 29, 2020·Seminars in Cancer Biology·Vaishali AggarwalAnupam Bishayee
Jan 8, 2021·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Misaki YashimaItsuro Kazama
Mar 29, 2021·Food Research International·A RashidinejadS M Jafari

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