Simultaneous determination and speciation analysis of arsenic and chromium in iron supplements used for iron-deficiency anemia treatment by HPLC-ICP-MS

Talanta
Uenderson Araujo-BarbosaPilar Bermejo-Barrera

Abstract

This work proposes the use of high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for simultaneous speciation of arsenic and chromium in iron supplements used for the treatment of anemia. The sample preparation procedure recommended for the total determination of arsenic and chromium was established using acid digestion in a microwave assisted oven. For speciation analysis, however, the microwave-assisted extraction procedure involved the use of water as extraction solvent at 90°C for 30min. The chromatographic separation was performed using a mobile phase containing 1.0mM tetrabutylammonium hydroxide (TBAH), 0.7mM ethylenediaminetetraacetic acid (EDTA) and 5% methanol at pH 7.2. Helium was used in the collision cell for elimination of the interferences. Under optimized conditions, the separation and detection of the As(III), As(V), Cr(III) and Cr(VI) species can be performed in 5min, permitting their quantification with the external calibration technique with standards prepared in the mobile phase. The limits of quantification obtained were 0.008, 0.010, 0.5 and 0.14µgg-1, for As(III), As(V), Cr(III) and Cr(VI), respectively. The accuracy of the method was evaluated and ...Continue Reading

Citations

Jun 24, 2017·Critical Reviews in Analytical Chemistry·Heena RekhiAshok Kumar Malik
Sep 11, 2019·Free Radical Research·João Guilherme CostaAna Sofia Fernandes
Mar 9, 2021·Critical Reviews in Analytical Chemistry·Mónica Gisel Arellano-SánchezMélanie Mignot
Jun 3, 2021·Talanta·María Vanesa Taboada-LópezAntonio Moreda-Piñeiro

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