Simultaneous determination of 11 aconitum alkaloids in human serum and urine using liquid chromatography-tandem mass spectrometry

Legal Medicine
Kiyotaka UsuiMasato Funayama

Abstract

A liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of four aconitines (aconitine, mesaconitine, hypaconitine, jesaconitine) and seven of their hydrolysis products (benzoylmesaconine, benzoylhypaconine, 14-O-anisoylaconine, benzoylaconine, aconine, mesaconine, hypaconine) in human serum and urine samples. Extraction was undertaken using a mixed-mode cation-exchange cartridge (OASIS MCX), and D(5)-aconitine was used as an internal standard. Separation of aconitum alkaloids was carried out using an L-column ODS with the mobile phase consisting of 10mM ammonium formate and methanol. The intra- and inter-day precisions were 0.3% to 9.9% and 3.2% to 12.8%, respectively. Intra- and inter-day accuracies were -14.1% to 7.3%, and -10.6% to 8.3%, respectively. The limit of detection and limit of quantification of analytes were 0.04-0.38 ng/mL and 0.12-1.15 ng/mL respectively. This method was applied in an autopsy case and successfully detected aconitines and their metabolites as well as some anti-psychiatric drugs.

References

Jun 1, 1992·The Tohoku Journal of Experimental Medicine·Y OhnoY Suzuki
Aug 15, 1996·Forensic Science International : Synergy·N YoshiokaM Mizugaki
Oct 7, 1998·Progress in Neurobiology·A Ameri
Jan 28, 2005·International Journal of Legal Medicine·J BeikeH Köhler
Sep 2, 2005·Toxicon : Official Journal of the International Society on Toxinology·Hong-Gui ZhangHan-Qi Zhang
Jan 31, 2006·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Fabienne MoritzChristophe Girault
Oct 6, 2006·International Journal of Legal Medicine·An A Van LandeghemMichel H A Piette
Jun 21, 2007·Journal of Analytical Toxicology·Yuji FujitaShigeatsu Endo
Feb 21, 2008·Journal of Forensic Sciences·Ravi PullelaEdward W Randell
Aug 5, 2009·Journal of Ethnopharmacology·Judith SinghuberBrigitte Kopp
Apr 1, 2010·Basic & Clinical Pharmacology & Toxicology·Antoine StrzeleckiMarc Clavel

❮ Previous
Next ❯

Citations

May 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fangyuan GaoGuorong Fan
Nov 8, 2012·Forensic Science International : Synergy·Hisae NiitsuKoji Dewa
Feb 24, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Min ZhangXiao-Bo Li
Jul 15, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Panpan ZhangLeiming Ren
Jul 17, 2019·BMC Research Notes·Masashi KitamuraYoshiaki Shirataki
Dec 6, 2019·Forensic Science, Medicine, and Pathology·Yong Soo ChoJoo-Young Na
Sep 7, 2017·Journal of Analytical Toxicology·Shaohua Li, Dawei Chen
Apr 9, 2018·Forensic Sciences Research·Xiangting GaoShaohua Zhu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.