Abstract
A liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of four aconitines (aconitine, mesaconitine, hypaconitine, jesaconitine) and seven of their hydrolysis products (benzoylmesaconine, benzoylhypaconine, 14-O-anisoylaconine, benzoylaconine, aconine, mesaconine, hypaconine) in human serum and urine samples. Extraction was undertaken using a mixed-mode cation-exchange cartridge (OASIS MCX), and D(5)-aconitine was used as an internal standard. Separation of aconitum alkaloids was carried out using an L-column ODS with the mobile phase consisting of 10mM ammonium formate and methanol. The intra- and inter-day precisions were 0.3% to 9.9% and 3.2% to 12.8%, respectively. Intra- and inter-day accuracies were -14.1% to 7.3%, and -10.6% to 8.3%, respectively. The limit of detection and limit of quantification of analytes were 0.04-0.38 ng/mL and 0.12-1.15 ng/mL respectively. This method was applied in an autopsy case and successfully detected aconitines and their metabolites as well as some anti-psychiatric drugs.
References
Jun 1, 1992·The Tohoku Journal of Experimental Medicine·Y OhnoY Suzuki
Aug 15, 1996·Forensic Science International : Synergy·N YoshiokaM Mizugaki
Feb 1, 1997·Planta medica·K ItoM Mizugaki
Apr 11, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·H OhtaN Tsunoda
Jul 29, 1998·Journal of Analytical Toxicology·M MizugakiK Kurasawa
Oct 7, 1998·Progress in Neurobiology·A Ameri
Aug 5, 2000·Journal of Analytical Toxicology·K ItoM Mizugaki
Aug 26, 2003·Legal Medicine·Makiko HayashidaYoukichi Ohno
Sep 11, 2003·Analytical Chemistry·B K MatuszewskiC M Chavez-Eng
Dec 21, 2004·Forensic Science International : Synergy·Kentaro WadaYoukichi Ohno
Jan 28, 2005·International Journal of Legal Medicine·J BeikeH Köhler
Sep 2, 2005·Toxicon : Official Journal of the International Society on Toxinology·Hong-Gui ZhangHan-Qi Zhang
Jan 31, 2006·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Fabienne MoritzChristophe Girault
Oct 6, 2006·International Journal of Legal Medicine·An A Van LandeghemMichel H A Piette
Feb 1, 1996·Planta medica·K ItoM Mizugaki
Jun 21, 2007·Journal of Analytical Toxicology·Yuji FujitaShigeatsu Endo
Feb 21, 2008·Journal of Forensic Sciences·Ravi PullelaEdward W Randell
Sep 10, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fan ZhangYu-Quan Wei
Aug 5, 2009·Journal of Ethnopharmacology·Judith SinghuberBrigitte Kopp
Apr 1, 2010·Basic & Clinical Pharmacology & Toxicology·Antoine StrzeleckiMarc Clavel
Citations
Jan 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Hua YangPing Li
Jun 8, 2014·Forensic Science International : Synergy·Luca AnzillottiSabina Strano-Rossi
Oct 21, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pei-Min DaiZhong-Qiu Liu
May 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fangyuan GaoGuorong Fan
Nov 8, 2012·Forensic Science International : Synergy·Hisae NiitsuKoji Dewa
Jun 12, 2013·Drug Testing and Analysis·Wolfgang BickerThomas Keller
Dec 18, 2014·Biomedical Chromatography : BMC·Hai ZhangYifeng Chai
Sep 12, 2015·Drug Testing and Analysis·Hai ZhangGuoqing Zhang
May 6, 2014·Journal of Ethnopharmacology·Hai ZhangYifeng Chai
May 28, 2013·Journal of Ethnopharmacology·Wen-Wen PengBao-Chang Cai
Feb 24, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Min ZhangXiao-Bo Li
Mar 24, 2016·Journal of Separation Science·Xiaozhong WangYongri Jin
Jul 15, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Panpan ZhangLeiming Ren
Mar 5, 2020·Natural Product Reports·Yong ShenDa-Ke Zhao
Jul 17, 2019·BMC Research Notes·Masashi KitamuraYoshiaki Shirataki
Dec 6, 2019·Forensic Science, Medicine, and Pathology·Yong Soo ChoJoo-Young Na
Sep 7, 2017·Journal of Analytical Toxicology·Shaohua Li, Dawei Chen
Apr 9, 2018·Forensic Sciences Research·Xiangting GaoShaohua Zhu