Simultaneous determination of 16 polycyclic aromatic hydrocarbons in source water and tap water by performance liquid chromatography with ultraviolet detector tandem fluorescence detector combined with solid phase extraction

Wei sheng yan jiu = Journal of hygiene research
Junlin WangJianlong Han

Abstract

To establish a rapid method for simultaneous determination of 16 polycyclic aromatic hydrocarbons(PAHs) in source water and tap water by performance liquid chromatography(HPLC) with ultraviolet detector(UV) tandem fluorescence detector(FLR). Source water was filtered by GF/C glass fiber filters and tap water were added ascorbic acid of 60 mg per liter to remove the residual chlorine when sampling. 500 mL water sample were sampled and adjusted pH 2 with phosphoric acid, then 10 mL methanol were added. Then samples were concentrated by styrene stilbene polymer solid phase extraction column, after loading samples, 50 percent methanol aqueous solution adjust pH 2 were used for washing bottle and the washed solution were continuum loaded. Then 80 percent methanol aqueous solution was used for removing impurity interference and elution with dichloromethane. The eluent was nitrogen blow to near dry after adding 100 μL 10 percent tween-20 methanol solutions(m/V). Acetonitrile was used for reconstitution, and then separated by PAH chromatography column using acetonitrile and pure water at gradient elution, and detected by UV tandem FLR detector. The linear ranges of 16 PAHs were 0. 5 to 500 ng/mL and the correlation coefficients were gr...Continue Reading

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