Simultaneous determination of 5-fluorouracil and its active metabolites in serum and tissue by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
M JungH J Buhr

Abstract

In order to observe the biodistribution of 5-fluorouracil (5-FU) and its main metabolites in different kinds of tissue (tumor, liver, kidney, spleen, mucosa, lungs, heart, peritoneum, pancreas) and serum according to various novel application forms, a simple and rapid method for the simultaneous determination of 5-FU and its active metabolites 5-fluorouridine (5-FUrd) and 5-fluoro-2'-deoxyuridine (5-FdUrd) has been established. Proteins in serum and tissue samples were precipitated by perchloric acid after addition of the internal standard 5-bromouracil. The compounds were separated using an ODS Hypersil (5 microm) column and detected by UV absorbance (254 nm). Specificity, linearity, reproducibility, intermediate precision and accuracy of the method were established. The lower limit of quantitation (LOQ) for the compounds in serum and various tissue samples was determined. Data on the recovery of the compounds and the internal standard are provided.

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Citations

Apr 13, 2010·Analytical and Bioanalytical Chemistry·Massimo Breda, Simona Barattè
Jun 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Federico CasaleMario Eandi
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hao-jie LuQing-yu Ou
Jul 12, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P CorrealeG Giorgi
May 13, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A AnithaR Jayakumar
Apr 18, 2008·The Journal of Pharmacy and Pharmacology·Ziyaur RahmanMichael A Repka
Mar 16, 2021·Journal of Pharmaceutical Analysis·Christelle MachonJérôme Guitton
Jun 22, 2005·Journal of Pharmaceutical and Biomedical Analysis·Rossella PisanoChristopher A James

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