Oct 1, 1975

Simultaneous determination of 5'-nucleotidase and alkaline phosphatase activities in serum

Zeitschrift Für Klinische Chemie Und Klinische Biochemie
A A El-Aaser, M M El-Merzabani

Abstract

A simple method is described for the simultaneous determination of alkaline phosphatase (EC 3.1.3.1) and 5'-nucleotidase (EC 3.1.3.5) in serum. The method is based on the determination of inorganic phosphorus released by the action of the two enzymes on adenosine-5'-monophosphate at pH 9.5 (200 mmol/l tris-buffer) in the presence and absence of L-cysteine. This amino acid at a concentration of 2--10 mmol/l was found to be a specific inhibitor for alkaline phosphatase but with no effect on 5'-nucleotidase activity.

  • References11
  • Citations6

References

Mentioned in this Paper

Nucleotidases
NT5E
Nickel
Isocyanides
Alkaline Phosphatase
Kidney
Fluorides
Phosphaden
Intestines
Hydrogen-Ion Concentration

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