Simultaneous determination of all-trans-, 13-cis- and 9-cis-retinoic acid, their 4-oxo metabolites and all-trans-retinol in human plasma by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Applications
C LanversJ Boos

Abstract

All-trans-retinoic acid (all-trans-RA) and 13-cis-retinoic acid (13-cis-RA), due to their effects on cell differentiation, proliferation and angiogenesis, improved treatment results in some malignancies. Pharmacokinetic studies of all-trans-RA and 13-cis-RA along with monitoring of retinoic acid metabolites may help to optimize retinoic acid therapy and to develop new effective strategies for the use of retinoic acids in cancer treatment. Therefore, we developed a HPLC method for the simultaneous determination in human plasma of the physiologically important retinoic acid isomers, all-trans-, 13-cis- and 9-cis-retinoic acid, their 4-oxo metabolites, 13-cis-4-oxoretinoic acid (13-cis-4-oxo-RA) and all-trans-4-oxoretinoic acid (all-trans-4-oxo-RA), and Vitamin A (all-trans-retinol). Analysis was performed on a silica gel column with UV detection at 350 nm using a binary multistep gradient composed of n-hexane, 2-propanol and glacial acetic acid. For liquid-liquid extraction a mixture of n-hexane, dichloromethane and 2-propanol was used. The limits of detection were 0.5 ng/ml for retinoic acids and 10 ng/ml for all-trans-retinol. The method showed good reproducibility for all components (within-day C.V.: 3.02-11.70%; day-to-day C....Continue Reading

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Citations

Mar 26, 2003·Medical and Pediatric Oncology·Claudia LanversJoachim Boos
Aug 20, 2014·Annals of the New York Academy of Sciences·Frank T WieringaJacques Berger
Jul 23, 2013·Food Chemistry·Mark SykesRosemary A Smith
Sep 26, 2007·BMC Public Health·Rongwang YangZhengyan Zhao
May 6, 2008·Nutrition Journal·Kim J SpaccarotellaTerryl J Hartman
Sep 11, 2010·PloS One·Michaël Boele van HensbroekMalcolm E Molyneux
Feb 9, 2008·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Menghua LuoThomas R Ziegler
Mar 3, 2017·Journal of Food Science·Eileen B YehMaryAnne Drake
Jul 2, 2002·Journal of Lipid Research·Peter McCafferyM David Ullman
May 12, 2006·Journal of Neurobiology·Thomas E Gundersen
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C LanversJ Boos

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