Simultaneous determination of amitraz and its metabolite in human serum by monolithic silica spin column extraction and liquid chromatography-mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Takeshi SaitoSadaki Inokuchi

Abstract

A simple, rapid, sensitive, and specific liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the quantification of amitraz and its metabolite in human serum. Both the compounds were extracted using monolithic silica spin columns with acetonitrile. The chromatographic separation was performed on a reverse-phase C(18) column with a mobile phase of 10 mM ammonium formate-acetonitrile. The protonated analyte was quantitated in positive ionization by mass spectrometry. The method was validated over the concentration range of 25-1000 ng/ml for amitraz and its metabolite in human serum. For both compounds, the limit of detection was 5 ng/ml. The method was applied to serum samples taken from an attempted suicide patient, and only small volumes of serum were required for the simultaneous determination of these compounds.

Citations

Sep 21, 2010·Analytical and Bioanalytical Chemistry·Li XuYu-Qi Feng
Feb 6, 2013·Analytical and Bioanalytical Chemistry·Dustin R Bunch, Sihe Wang
Jun 22, 2013·Journal of Pharmaceutical and Biomedical Analysis·Tarang NemaPaul C Ho
Oct 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takeshi SaitoSadaki Inokuchi
Apr 18, 2013·Bioanalysis·Akira Namera, Takeshi Saito
Oct 16, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hongxia LiXiaohong Yang
Sep 5, 2015·Bioanalysis·Akira Namera, Takashi Saito
Jul 30, 2015·Journal of Pharmaceutical and Biomedical Analysis·Erina TamashimaHitoshi Nohta
Aug 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Erina KohyamaTetsuro Ito
Mar 12, 2011·Journal of Separation Science·Akira NameraShota Miyazaki
May 21, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Manish Kumar SharmaPinaki Sengupta
Dec 8, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigenori OtaShuichi Yamamoto

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