PMID: 7420289Oct 1, 1980

Simultaneous determination of aspirin and salicylic acid in bulk aspirin and in plain, buffered, and enteric-coated tablets by high-pressure liquid chromatography with UV and fluorescence detectors

Journal of Pharmaceutical Sciences
R D Kirchhoefer


A quantitative high-pressure liquid chromatographic method that uses a reversed-phase column coupled to UV and fluorescence detectors was developed to determine aspirin and salicylic acid in bulk aspirin and in plain, buffered, and enteric-coated tablets. The aspirin was dissolved, filtered, and injected into the chromatograph. The UV absorbance of aspirin was determined at 254 nm, and the fluorescence of salicylic acid was measured at 425 nm. Excipients and impurities did not interfere. Recoveries of 100% were obtained for aspirin and salicylic acid from simulated tablet formulations. Results obtained by the USP XIX procedure and the proposed method were compared. The coefficient of variation for the aspirin analysis was 0.59%; for salicylic acid, it was 1.69%. The rate of hydrolysis of aspirin to salicylic acid in the solvents used was < 0.05%/hr.


Aug 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·T J McCormickF J Diana
May 1, 1982·Journal of Pharmaceutical Sciences·C D Pfeiffer, J W Pankey
Jul 1, 1982·The Journal of Pharmacy and Pharmacology·P MazzeoF Segnalini

Related Concepts

High Pressure Liquid Chromatography Procedure
Salicylic Acids
Fluorescence Spectroscopy
Spectrophotometry, Ultraviolet
Enteric Coated Tablet

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.