Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection

Journal of Chromatography. a
Baiqing YuanTianyan You

Abstract

A rapid and simple method was demonstrated for the analysis of atropine, anisodamine, and scopolamine by nonaqueous capillary electrophoresis (NACE) coupled with electrochemiluminescence (ECL) and electrochemistry (EC) dual detection. The mixture of acetonitrile (ACN) and 2-propanol containing 1M acetic acid (HAc), 20mM sodium acetate (NaAc), and 2.5mM tetrabutylammonium perchlorate (TBAP) was used as the electrophoretic buffer. Although a short capillary of 18cm was used, the decoupler was not needed and the separation efficiency was good. The linear ranges of atropine, anisodamine, and scopolamine were 0.5-50, 5-2000, and 50-2000microM, respectively. For six replicate measurements of 100microM scopolamine, 15microM atropine, and 200microM anisodamine, the RSDs of ECL intensity, EC current, and migration time were less than 3.6%, 4.5%, and 0.3%, respectively. In addition, because the organic buffer was used, the working electrode (Pt) was not easily fouled and did not need reactivation. The method was also applied for the determination of these three alkaloids in Flos daturae extract.

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Citations

Dec 25, 2010·Analytical and Bioanalytical Chemistry·Longhua GuoGuonan Chen
Jun 12, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Huan YuTianyan You
Jul 3, 2013·Biomedical Chromatography : BMC·Szymon DziombaTomasz Bączek
Aug 9, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Szymon DziombaPiotr Kowalski
Nov 6, 2013·Science & Justice : Journal of the Forensic Science Society·Jorge SáizCarmen García-Ruiz
Jun 22, 2016·Journal of Fluorescence·Javad Abbasifar, Abdolraouf Samadi-Maybodi
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Dec 15, 2010·Drug Testing and Analysis·Jacqui L AdcockPaul S Francis
Jan 8, 2013·The Analyst·Ouissam RamdaniCraig E Banks
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcin GackowskiArtur Bogacz
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elke Aehle, Birgit Dräger
Dec 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Roberto Gotti

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