Simultaneous determination of biogenic monoamines in rat brain dialysates using capillary high-performance liquid chromatography with photoluminescence following electron transfer

Analytical Chemistry
Moon Chul JungStephen G Weber

Abstract

Simultaneous determination of biogenic monoamines such as dopamine, serotonin, and 3-methoxytyramine in brain is important in understanding neurotransmitter activity. This study presents a sensitive determination of biogenic monoamines in rat brain striatum microdialysates using capillary high-performance liquid chromatography with the photoluminescence following electron-transfer detection technique. Separation conditions were optimized by changing the concentration of an ion-interaction agent and the percentage of an organic modifier. The high concentration of ion-interaction agent enabled the amines as a class to be separated from interfering acids, but also made the separation very long. To shorten the separation time, 10% (v/v) acetonitrile was used as the organic modifier. Eight chromatographic runs during a 3-h period were analyzed in terms of retention times, peak heights, and peak widths. Chromatograms are very reproducible, with less than 1% changes in peak height over 3 h. Typical concentration detection limits at the optimum separation conditions were less than 100 pM for metabolic acids and approximately 200 pM for monoamines. The injection volume of the sample was 500 nL. Thus, the mass detection limits were less ...Continue Reading

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Citations

Jan 17, 2014·Journal of Chemical Biology·Gillian F WhyteRudiger Woscholski
Jul 19, 2008·Annual Review of Analytical Chemistry·Kristin N Schultz, Robert T Kennedy
Oct 6, 2009·Analytica Chimica Acta·Maura PerryRobert T Kennedy
Jul 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jolien Van SchoorsAnn Van Eeckhaut
Apr 22, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maki OkadaOsamu Inoue
Nov 2, 2017·Journal of Neuroscience Research·Akimasa IshidaHideki Hida
Jul 1, 2008·Critical Reviews in Analytical Chemistry·Teresa Cecchi
Apr 11, 2009·Analytical Chemistry·Jin-Young ParkSu-Moon Park
Nov 30, 2012·Analytical Chemistry·Siriwat SansukJulie V Macpherson

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