PMID: 11339288May 8, 2001Paper

Simultaneous determination of buprenorphine, norbuprenorphine, and buprenorphine-glucuronide in plasma by liquid chromatography-tandem mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
A Polettini, Marilyn A Huestis

Abstract

For the first time, an LC-MS-MS method has been developed for the simultaneous analysis of buprenorphine (BUP), norbuprenorphine (NBUP), and buprenorphine-glucuronide (BUPG) in plasma. Analytes were isolated from plasma by C18 SPE and separated by gradient RP-LC. Electrospray ionization and MS-MS analyses were carried out using a PE-Sciex API-3000 tandem mass spectrometer. The m/z 644-->m/z 468 transition was monitored for BUPG, whereas for BUP, BUP-d4, NBUP, and NBUP-d3 it was necessary to monitor the surviving parent ions in order to achieve the required sensitivity. The method exhibited good linearity from 0.1 to 50 ng/ml (r2> or =0.998). Extraction recovery was higher than 77% for BUPG and higher than 88% for both BUP and NBUP. The LOQ was established at 0.1 ng/ml for the three analytes. The method was validated on plasma samples collected in a controlled intravenous and sublingual buprenorphine administration study. Norbuprenorphine-glucuronide was also tentatively detected in plasma by monitoring the m/z 590-->m/z 414 transition.

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Citations

Jul 27, 2001·Journal of Mass Spectrometry : JMS
Feb 21, 2013·Journal of Analytical Toxicology·Thomas BergDag Helge Strand
Feb 15, 2013·Journal of Analytical Toxicology·Marcela Nogueira Rabelo AlvesAldo Polettini
Mar 9, 2013·Journal of Analytical Toxicology·M A BirchR J Flanagan
Apr 25, 2007·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M L JensenA Somogyi
Jan 6, 2016·Journal of Pharmaceutical and Biomedical Analysis·Yongzhen LiuChristian Heidbreder
Feb 11, 2011·Forensic Science International : Synergy·Tor SeldénRobert Kronstrand
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura MercoliniMaria Augusta Raggi
Dec 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria Esther Rodriguez-RosasIrving W Wainer
Dec 31, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yan ChangElinore F McCance-Katz
Jun 28, 2003·Natural Product Reports·Kenneth W Bentley
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marta TikhomirovTomasz Śniegocki
Dec 23, 2019·Journal of Pharmaceutical and Biomedical Analysis·Camille CohierOlivier Roussel

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