Simultaneous determination of caffeine, paracetamol, and ibuprofen in pharmaceutical formulations by high-performance liquid chromatography with UV detection and by capillary electrophoresis with conductivity detection

Journal of Separation Science
Rafael Rodrigues CunhaEduardo Mathias Richter

Abstract

Paracetamol, caffeine and ibuprofen are found in over-the-counter pharmaceutical formulations. In this work, we propose two new methods for simultaneous determination of paracetamol, caffeine and ibuprofen in pharmaceutical formulations. One method is based on high-performance liquid chromatography with diode-array detection and the other on capillary electrophoresis with capacitively coupled contactless conductivity detection. The separation by high-performance liquid chromatography with diode-array detection was achieved on a C18 column (250×4.6 mm(2), 5 μm) with a gradient mobile phase comprising 20-100% acetonitrile in 40 mmol L(-1) phosphate buffer pH 7.0. The separation by capillary electrophoresis with capacitively coupled contactless conductivity detection was achieved on a fused-silica capillary (40 cm length, 50 μm i.d.) using 10 mmol L(-1) 3,4-dimethoxycinnamate and 10 mmol L(-1) β-alanine with pH adjustment to 10.4 with lithium hydroxide as background electrolyte. The determination of all three pharmaceuticals was carried out in 9.6 min by liquid chromatography and in 2.2 min by capillary electrophoresis. Detection limits for caffeine, paracetamol and ibuprofen were 4.4, 0.7, and 3.4 μmol L(-1) by liquid chromatogra...Continue Reading

References

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Citations

Dec 8, 2015·Analytical Chemistry·Rachel K HarstadMichael T Bowser
Apr 10, 2016·Electrophoresis·Andrea BeutnerFrank-Michael Matysik
Oct 25, 2016·Journal of Chromatography. a·Michelle M A C RibeiroEduardo M Richter
Feb 9, 2017·Biomedical Chromatography : BMC·Abdalla A ElbashirHassan Y Aboul-Enein
May 23, 2018·Journal of Separation Science·Brenda Maria de Castro CostaEduardo Mathias Richter
Oct 30, 2015·Journal of Separation Science·Marína Rudašová, Marián Masár
Sep 10, 2020·Electrophoresis·Roman Řemínek, František Foret
Aug 13, 2017·Electrophoresis·Yuanyuan LiuChao Yan
Aug 10, 2019·Applied Biochemistry and Biotechnology·Jie QiaoNingbo Li
Jul 7, 2018·International Journal of Analytical Chemistry·Rimadani PratiwiAliya Nur Hasanah
Apr 23, 2021·Journal of Separation Science·Aliceana Almeida do PradoEduardo Mathias Richter

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