Simultaneous determination of capecitabine and its metabolite 5-fluorouracil by column switching and liquid chromatographic/tandem mass spectrometry

Journal of Mass Spectrometry : JMS
Christoph SiethoffWinfried Wagner-Redeker

Abstract

A liquid chromatographic/tandem mass spectrometric method was developed and validated for the quantitation of capecitabine and its metabolite 5-fluorouracil in human plasma. The simultaneous determination of both analytes was achieved by a column switching method using a trapping column and two analytical columns with different stationary phases. Isocratic elution was used for the separation of capecitabine on a C18 column whereas 5-fluorouracil was separated using gradient elution on an non-polar carbon phase. The calibration curves were linear for both compounds with a correlation factor (R2) > 0.9993 for 5-fluorouracil and >0.9942 for capecitabine. The assay was validated in the concentration range 5.00-1000 ng ml(-1) for both compounds. The intra-day precision was better than 10% for 5-fluorouracil and better than 11% for capecitabine whereas the inter-day precision was better than 8% for 5-fluorouracil and better than 14% for capecitabine.

Citations

Dec 29, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maarten J DeenenJos H Beijnen
Feb 9, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M WagnerG Hopfgartner
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Delphine CarliJérôme Guitton
Dec 19, 2007·Rapid Communications in Mass Spectrometry : RCM·Juliann E KosovecJan H Beumer
Oct 4, 2011·Talanta·Susanne NussbaumerSandrine Fleury-Souverain
Feb 6, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Jalal Niazi SaeiHossein Karimian
Jun 15, 2005·Analytical Chemistry·R K Gilpin, L A Pachla

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