Simultaneous determination of citalopram and its metabolite in human plasma by LC-MS/MS applied to pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Tao JiangHongzhuan Chen

Abstract

A simple sensitive and robust method for simultaneous determination of citalopram and desmethylcitalopram was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS). A 200 microL aliquot of plasma sample was employed and deproteinized with methanol and desipramine was used as the internal standard. After vortex mixing and centrifugation, the supernatant was diluted with water (1:1, v/v) and then directly injected to analysis. Analytes were separated by a Zorbax XDB C(18) column with the mobile phase composed of acetonitrile and water (30:70, v/v) with 0.25% formic acid and monitored in MRM mode using a positive electrospray source with tandem mass spectrometry detection. The total run time was 3.5 min. The dynamic range was 0.2-100 ng/mL for citalopram and 0.25-50 ng/mL for desmethylcitalopram, respectively. Compared to the best existing literatures for plasma samples, the same LOQ for CIT (0.5 ng/mL) and lower LOQ for DCIT (0.25 vs 5 ng/mL) were reached, and less sample preparation steps and runtime (3.5 vs 10 min) were taken for our method. Accuracy and precision was lower than 8% and lower than 11.5% for either target. Validation results and its application to the analysis of plasma samples after oral admi...Continue Reading

References

Dec 30, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M KoselP Baumann
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Sep 17, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Bang-Ning YuHong-Hao Zhou
Sep 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Constantinos PistosJulia Atta-Politou
Feb 23, 2008·Critical Reviews in Clinical Laboratory Sciences·Sarah M R WilleWilly E E Lambert

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