Simultaneous determination of codeine and chlorpheniramine in human plasma by capillary column gas chromatography

Journal of Chromatography
K MasumotoS Hayashi

Abstract

A specific and highly sensitive capillary column gas chromatographic method was developed for the simultaneous determination of codeine and chlorpheniramine in human plasma. The method involves a solvent extraction and analysis by capillary column gas chromatography on a cross-linked 50% phenylmethyl silicone fused-silica capillary column with flame thermionic detection. A 10% solution of n-butanol in toluene was used as extraction medium and pyrilamine was used as internal standard. Reproducibility, linearity of calibration curves and specificity were all satisfactory with both drugs. The plasma concentration of codeine and chlorpheniramine could be measured at levels down to 0.9 ng/ml as codeine phosphate and 0.4 ng/ml as chlorpheniramine maleate, respectively. The method was applied to plasma samples from normal volunteers, and was confirmed to be adequate for biopharmaceutical and pharmacokinetic studies.

References

May 1, 1978·Journal of Medicinal Chemistry·J I DeGrawD S Berkowitz
Jul 1, 1978·Clinical Pharmacology and Therapeutics·J W FindlayR M Welch
Dec 1, 1974·Journal of Pharmaceutical Sciences·S Hanna, A Tang
Dec 1, 1970·Analytical Chemistry·E TownleyP Kabasakalian
Nov 1, 1984·Journal of Pharmaceutical Sciences·V Nitsche, H Mascher
Mar 1, 1984·Chemical & Pharmaceutical Bulletin·K MasumotoT Nagai

❮ Previous
Next ❯

Citations

Nov 4, 1994·Journal of Chromatography. B, Biomedical Applications·M YamaguchiT Yashiki
Jul 26, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takeshi TakagakiYoshiaki Terauchi
Dec 4, 2009·Journal of Pharmaceutical and Biomedical Analysis·Adnan ManassraFuad Al-Rimawi
Aug 14, 2009·Journal of Forensic Sciences·Barry K Logan
Mar 6, 2015·Journal of Analytical Methods in Chemistry·Robert PiechBeata Paczosa-Bator
Apr 3, 2016·Biomedical Chromatography : BMC·Maria KatselouSotiris Athanaselis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.