Simultaneous determination of corynoline and acetylcorynoline in human urine by LC-MS/MS and its application to a urinary excretion study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ruijuan LiuLi Ding

Abstract

Corynoline and acetycorynoline, the major active components derived from Corydalis bungeana Herba, showed multiple pharmacological activities. However, quantification of the two compounds in human urine has not been reported. A simple liquid chromatography with tandem mass spectrometry method for the simultaneous determination of corynoline and acetycorynoline in human urine has been developed and fully validated. The analytes were extracted from urine samples by simple liquid-liquid extraction. Chromatographic separation was achieved on a Hedera ODS-2C18 column with the mobile phase of water (containing 0.5% formic acid) and acetonitrile (28:72, v/v) at a flow rate of 0.4mL/min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring via an electrospray ionization source in positive mode. The monitored ion transitions were m/z 368.1→289.1 for corynoline, m/z 410.2→289.2 for acetycorynoline and m/z 380.2→243.2 for donepezil (internal standard), respectively. The calibration curves were linear (correlation coefficients>0.9970) over the concentration ranges of 3.0-3000pg/mL for corynoline and 3.0-1000pg/mL for acetycorynoline. The established method was highly sensitive with the lower limit of quant...Continue Reading

References

Oct 27, 2004·Phytochemistry·Chen XieMonique S J Simmonds
Mar 15, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H LvH Cao
May 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chunjuan YangGaofeng Liu
Jan 27, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ju-Hee RyuKyung-Tae Lee
Nov 23, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yao WuLi Ding

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