Abstract
Two-component mixtures of dorzolamide hydrochloride and timolol maleate were assayed by first derivative and ratio derivative spectrophotometric methods. The first method, derivative spectrophotometry, by the zero-crossing measurements, was used due to the drugs closely overlapping absorption spectra. Linear calibration graphs of first derivative values at 250.3 nm for dorzolamide hydrochloride and 315.8 nm for timolol maleate. The second method, is based on ratio first derivative spectrophotometry, the amplitudes in the first derivative of the ratio spectra at 242.9 and at 223.5 nm were selected to determine dorzolamide and timolol maleate in the binary mixture. Calibration graphs were established for 8.0-30.0 microg ml(-1) for dorzolamide hydrochloride and 3.0-24.6 microg ml(-1) for timolol maleate in binary mixture. Good linearity, precision and selectivity were found, and the proposed methods were applied successfully to the pharmaceutical dosage from containing the above-mentioned drug combination without any interference by the excipients. Vierordt's method was also developed for a comparison method.
References
Apr 1, 1988·Journal of Chromatography·D J Mazzo, P A Synder
Sep 21, 1984·Journal of Chromatography·D J Mazzo
Apr 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·M L ConstanzerB K Matuszewski
Citations
Nov 19, 2003·Analytical Biochemistry·Nevin Erk
Mar 13, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hayam Mahmoud LotfyYasmin Rostom Omran
May 9, 2012·Journal of Chromatographic Science·Nitish SharmaA Malleswara Reddy
Jan 6, 2006·Chemical & Pharmaceutical Bulletin·Effat SouriAlma Afshari
Feb 21, 2016·Annales pharmaceutiques françaises·T S BelalA H Abo-Gharam
Jun 17, 2009·Journal of Pharmaceutical Sciences·Mohsen I AfounaAdnan Al-Marzoqi
Apr 26, 2005·Il Farmaco·J S MillershipD Donnelly
Apr 19, 2015·Journal of Pharmaceutical and Biomedical Analysis·Carolina BoieroJuan M Llabot
Dec 6, 2016·International Journal of Molecular Sciences·Nawal Ahmad Alarfaj, Maha Farouk El-Tohamy
Aug 28, 2014·Talanta·Abdel-Maaboud Ismail MohamedHeba Salah Mousa
Jun 25, 2010·Journal of Separation Science·Mojtaba ShamsipurAssem Abdollahpour
Jun 30, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Abdel-Aziz Y El-Sayed, Najeb A El-Salem
Oct 3, 2018·Journal of Analytical Methods in Chemistry·Boovizhikannan ThangabalanTadele Eticha
Apr 1, 2016·Journal of Food and Drug Analysis·Mohamed Walash, Rania El-Shaheny
Aug 10, 2016·Nanotechnology·Amirmostafa AmirjaniSaeed Hesaraki
Dec 1, 2009·Journal of Drug Targeting·Sheetu WadhwaS P Vyas
Mar 27, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Srushti TambePurnima Amin
Feb 20, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Fadel WedianMakaseb S Mohammed
Apr 29, 2021·Drug Testing and Analysis·Alfredo Fabrizio Lo FaroFrancesco Paolo Busardò