PMID: 11916020Mar 28, 2002Paper

Simultaneous determination of eleven benzodiazepine hypnotics and eleven relevant metabolites in urine by column-switching liquid chromatography-mass spectrometry

Journal of Analytical Toxicology
Akihiro MikiHitoshi Tsuchihashi

Abstract

An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase extraction technique was employed to enhance sensitivity and eliminate tedious sample pretreatment. The combination of an N-vinylacetamide-containing hydrophilic polymer online extraction column and a C18 semi-micro LC column provided the successful separations and MS determinations of the 11 benzodiazepines and 11 relevant metabolites tested in this study. The detection limits ranged 2-10 ng/mL and 10-50 ng/mL in the SIM and full-scan modes, respectively, using 50 microL of urine. The calibration curves were linear up to 500 ng/mL for all these analytes in the SIM mode. The present method provided successful forensic applications for the proof of benzodiazepine administration.

Citations

Oct 23, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hei Hwa LeeBai Hsiun Chen
Sep 14, 2014·Journal of Analytical Toxicology·Ayodele A MorrisGregory L McIntire
Aug 12, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ryh-Nan PanLi-Heng Pao
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Apr 22, 2004·European Journal of Mass Spectrometry·Hazel M RiveraPeter C Stockham
Jul 1, 2009·Bioanalysis·Victoria F SamanidouIoannis N Papadoyannis
Sep 5, 2017·International Journal of Speech-language Pathology·Rachelle PittTrevor Russell
Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein

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