Simultaneous determination of evodiamine and its four metabolites in rat plasma by LC-MS/MS and its application to a pharmacokinetic study

Biomedical Chromatography : BMC
Cuiyun WangJie Yang

Abstract

A simple and sensitive liquid chromatography tandem mass spectrometry method was validated for simultaneous quantification of evodiamine and its metabolites 10-hydroxyevodiamine (M1), 18-hydroxyevodiamine (M2), 10-hydroxyevodiamine-glucuronide (M3) and 18-hydroxy- evodiamine-glucuronide (M4) in rat plasma for the first time. The analytes were extracted with acetonitrile and separated on a C18 column within 3 min. The detection was achieved in positive selected reaction monitoring mode with precursor-to-product transitions at m/z 304.1 → 161.1 for evodiamine, m/z 320.1 → 134.1 for M1, m/z 320.1 → 150.1 for M2, m/z 496.2 → 134.1 for M3, m/z 496.2 → 171.1 for M4 and m/z 349.2 → 305.1 for camptothecin (internal standard). The linearity was evident over the tested concentration ranges with correlation coefficients >0.9991. The lower limits of quantification for evodiamine, M1, M2, M3 and M4 were 0.1, 0.1, 0.1, 0.25 and 0.25 ng mL-1 , respectively. Extraction recoveries and matrix effects of the analytes were within the ranges of 84.51-97.21 and 90.13-103.30%, respectively. The accuracy (relative error) ranged from -8.14 to 7.23% while the intra- and inter-day precisions (relative standard deviation) were < 9.31%. The validated assay...Continue Reading

References

Dec 1, 1978·Journal of Pharmacokinetics and Biopharmaceutics·K YamaokaT Uno
May 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Junlin Jiang, Changping Hu
Mar 10, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xiaobei PanS W Annie Bligh
Feb 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ping QianXiu-Wei Yang

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Citations

Sep 11, 2020·The American Journal of Chinese Medicine·Shuang LiDong Shang
Aug 4, 2019·Pharmaceutics·Robert AncuceanuVlad Negulescu
Dec 20, 2021·Chemical Biology & Drug Design·Weihang GuoChangkuo Zhao

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