Simultaneous determination of four coccidiostats in eggs and broiler meat: validation of an LC-MS/MS method

Food Additives and Contaminants
Mervi Rokka, Kimmo Peltonen

Abstract

A published confirmatory method for the quantitative determination of four ionophoric coccidiostats (lasalocid, monensin, salinomycin and narasin) in eggs and broiler meat has been further developed. It is proposed for replacement of liquid chromatography methods previously used in analysis of ionophoric coccidiostats. The samples were extracted with acetonitrile and purified on a silica solid phase extraction column. Purified samples were analysed by liquid chromatography-mass spectrometry and the method, was validated according to the Commission Decision 2002/657/EC. The validation parameters selectivity, linearity, specificity, precision, recovery, decision limit (CCalpha) and detection capability (CCbeta) were determined. The recoveries of coccidiostats analysed ranged from 64-99% in eggs and 62-100% in broiler meat. CCalpha varied from 0.8-1.4 microg/kg in eggs and from 1.5-2.5 microg/kg in broiler meat. CCbeta varied from 0.9 microg/kg to 2.0 microg/kg in eggs and from 1.7-3.2 microg/kg in broiler meat.

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Citations

May 2, 2014·Food Additives & Contaminants. Part B, Surveillance·Nina BilandžićBožica Solomun Kolanović
Mar 29, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·I KožárováS Tkáčiková
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Mar 14, 2013·BioMed Research International·Mervi RokkaKimmo Peltonen
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Jul 6, 2014·BioMed Research International·Bruno Alves RochaMarilda das Dores Assis
Oct 19, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bruno A RochaAnderson R M de Oliveira
May 3, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Rosa Elvira GavilánAlberto Cepeda
Apr 13, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Konrad PietrukAndrzej Posyniak
Jan 3, 2007·Journal of Mass Spectrometry : JMS
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Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo WangGuojun Dai
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Mar 2, 2021·Food Research International·Felipe Rodrigues Nogueira SilvaAdriana Pavesi Arisseto-Bragotto

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Methods Mentioned

BETA
solid phase extraction

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