Simultaneous determination of four plant hormones in bananas by molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography

The Analyst
Hongyuan YanGengliang Yang

Abstract

A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection was developed for the simultaneous isolation and determination of four plant hormones including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) in banana samples. The new molecularly imprinted microspheres (MIMs) prepared by aqueous suspension polymerization using 3-hydroxy-2-naphthoic acid and 1-methylpiperazine as mimic templates performed with high selectivity and affinity for the four plant hormones, and applied as selective sorbents of solid-phase extraction could effectively eliminate the interferences of the banana matrix. Good linearity was obtained in a range of 0.04-4.00 μg g(-1) and the recoveries of the four plant hormones at three spiked levels ranged from 78.5 to 107.7% with the relative standard deviations (RSD) of less than 4.6%. The developed MISPE-HPLC protocol obviously improved the selectivity and eliminated the effect of template leakage on quantitative analysis, and could be applied for the determination of plant hormones in complicated biological samples.

References

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Citations

Mar 25, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fengxia QiaoMengge Wang
Apr 13, 2013·Journal of Experimental Botany·David A KorasickLucia C Strader
May 9, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xiangxiang Liu, Yiqun Wan
Mar 5, 2016·Chemical Society Reviews·Lingxin ChenJinhua Li
Dec 26, 2015·Analytica Chimica Acta·Sara PorfírioParastoo Azadi
Nov 27, 2016·Journal of Separation Science·Guizhen LiKyung Ho Row
Oct 23, 2020·Food Science and Biotechnology·Song ZhuYue Li

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