Simultaneous determination of four tobacco-specific N-nitrosamines in mainstream smoke for Chinese Virginia cigarettes by liquid chromatography-tandem mass spectrometry and validation under ISO and "Canadian intense" machine smoking regimes

Analytica Chimica Acta
Wei XiongQingyuan Hu

Abstract

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining four tobacco-specific N-nitrosamines (TSNAs) in mainstream smoke from Chinese Virginia cigarettes was developed. Mainstream cigarette smoke particulate matter was collected on a Cambridge filter pad, further extracted using 100 mM ammonium acetate after 100 microL internal standard addition, and subsequently analyzed with LC-MS/MS. The limit of detection for NNN, NNK, NAT and NAB were 0.006, 0.013, 0.003 and 0.021 ng mL(-1) respectively, with a linear calibration range spanning 1-200 ng mL(-1). Intra- and inter-day precision for four TSNAs ranged from 3.3% to 8.5% and 2.3% to 10.1%; recovery was between 89.1% and 104.9% for Chinese Virginia cigarettes. The proposed method was applied to evaluate TSNAs yields for 39 commercially available cigarettes in Chinese market under ISO and "Canadian intense" machine smoking regimes, on the ground that it comes closest to representing smoke deliveries from human smoking. Total TSNAs emissions are more than double under the Canadian regime. TSNAs:nicotine ratios were used in our assay to show any differences in yield from different brands. TSNAs:nicotine levels show more than a 10-fold difference across bran...Continue Reading

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Mar 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dominique KavvadiasMike McEwan
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Citations

May 11, 2016·International Journal of Cancer. Journal International Du Cancer·Katrina YershovaIrina Stepanov
Aug 9, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Emily C A StoneUNKNOWN International Association for the Study of Lung Cancer Tobacco Control Committee
Aug 11, 2020·Critical Reviews in Food Science and Nutrition·Yu BianXue-Song Feng
Dec 30, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Sungshim L ParkStephen S Hecht
Mar 6, 2021·Chemical Research in Toxicology·Selvin H EdwardsLiza Valentín-Blasini
Jan 16, 2014·Environmental Science & Technology·Minghuo WuXing-Fang Li

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