Simultaneous determination of gastric cancer biomarkers pepsinogen PGI/PGII using element tagged immunoassay coupled with inductively coupled plasma mass spectrometry detection.

Journal of Clinical Laboratory Analysis
Wencan JiangChengbin Wang

Abstract

In this study, a new immunoassay for the simultaneous determination of pepsinogen I (PGI) and pepsinogen II (PGII) in serum based on element labeling strategy coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection was proposed. The sandwich-type immunoassay was used to simultaneously detect PGI and PGII in serum. PGI and PGII were captured by anti-PGI and anti-PGII antibody immobilized on the magnetic beads and then banded with Eu3+ labeled anti-PGI detection antibody and Sm3+ labeled anti-PGII detection antibody, followed by ICP-MS detection. The linear correlation coefficient (R2 ) of PGI and PGII standard curves was .9938 and .9911, with the dynamic range of 0-200 ng/mL and 0-60 ng/mL, respectively. The limit of detection for PGI and PGII was 1.8 ng/mL and 0.3 ng/mL, respectively. The average recovery was 101.41% ± 6.74% for PGI and 101.47% ± 4.20% for PGII. Good correlations were obtained between the proposed method and CLIA (r = .9588 for PGI, r = .9853 for PGII). We established a mass spectrometry-based immunoassay for the simultaneous detection of PGI and PGII in a single analysis. The element tagged immunoassay coupled with ICP-MS detection has high sensitivity, accuracy, and specificity in clinica...Continue Reading

References

Jan 20, 2007·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kazumasa Miki
Jan 17, 2012·Scandinavian Journal of Gastroenterology·Lars AgréusJoseph Sung
Nov 26, 2013·Mass Spectrometry Reviews·Rui LiuYi Lv
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Nov 21, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eun-Jung ChoWon-Ki Min
Sep 25, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E C SmythUNKNOWN ESMO Guidelines Committee
Nov 24, 2017·Chemical Communications : Chem Comm·Gongwei SunXinrong Zhang
Sep 28, 2018·Nature Protocols·Guojun HanGarry P Nolan

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Methods Mentioned

BETA
sandwich immunoassay
ELISA

Software Mentioned

Origin
GraphPad Prism

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