Simultaneous determination of glucose, fructose, sucrose and sorbitol in the leaf and fruit peel of different apple cultivars by the HPLC-RI optimized method

Food Chemistry
Miuţa FilipAdela Halmagyi

Abstract

A high performance liquid chromatography method with refractive index detection (HPLC-RI), for simultaneous determination of glucose, fructose, sucrose and sorbitol in leaf and/or apple peel samples from nine apple (Malus domestica Borkh.) cultivars and rootstocks, originating from a germplasm collection, has been developed and validated. Box-Behnken design of response surface methodology was applied for the method optimization. The Carbosep Coregel 87H3 column was used under the optimum conditions predicted: mobile phase of H2SO4 0.005molL(-1) solution, flow rate of 0.3mLmin(-1) and column temperature of 35°C. The method was validated for linearity (R(2)>0.99), limits of detection (2.67-4.83μgmL(-1)) and quantification (8.9-16.1μgmL(-1)), precision (%RSD<5.05) and recovery (93.94-103.06%) and satisfactory results obtained. The sugars content varied across micropropagated plants in vitro, plants regenerated after cryostorage, growing trees in vivo, and fruit peel.

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Citations

Jul 30, 2016·Forensic Science International : Synergy·Jade B KillWanderson Romão
Aug 25, 2018·Journal of the Science of Food and Agriculture·Yonghong GeXihong Li
Sep 1, 2019·Journal of the Science of Food and Agriculture·Ting FangYuepeng Han
Aug 12, 2017·Topics in Current Chemistry·Leslie BrownKenneth R Seddon
Jun 21, 2020·Journal of the Science of Food and Agriculture·Weiwei ZhouXianyong Lin
Apr 21, 2017·Natural Product Research·Z XuH P V Rupasinghe
Oct 21, 2017·Critical Reviews in Analytical Chemistry·Marta Pokrzywnicka, Robert Koncki
Oct 23, 2019·World Journal of Microbiology & Biotechnology·Lin-Shang ZhangWen-Yong Lou
Dec 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Johannes Pitsch, Julian Weghuber
Jun 27, 2021·Scientific Reports·Preeyarad CharoensumranThanit Praneenararat
Aug 29, 2021·Plants·Iulian Eugen RusuSevastiţa Muste

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