Simultaneous determination of glucuronides of trimetozine in human urine by gas chromatography

Journal of Chromatography
K Kawahara, T Ofuji


A gas chromatographic method for the simultaneous determination of four glucuronides (metabolites) of trimetozine excreted in human urine is described. The method involves pretreatment of the urine specimen [i.e. removal of interfering substances by solvent extraction, desalting on an ion-exchange (Amberlite XAD-2) column], and permethylation of glucuronides by reaction with methylsulfinyl carbanion and methyl iodide. The permethylated derivatives were submitted to gas chromatographic separation on an OV-17 column, and their structures were investigated by subsequent gas chromatographic-mass spectrometric analysis. The minimum detectable concentration of each glucuronide is 5 micrograms/ml when 1 ml of urine is used. The utility of the present method is successfully demonstrated by determining the urinary concentration of four glucuronides following oral administration of trimetozine to human subjects.


Jan 1, 1984·Journal of Pharmaceutical and Biomedical Analysis·A Hulshoff, H Lingeman

Related Concepts

Chromatography, Gas-Liquid
Glucuronic Acids
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Chromatography, Gas-Liquid-Mass Spectrometry

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