Simultaneous determination of GTS-21 and its metabolite in rat plasma by high-performance liquid chromatography using solid-phase extraction

Journal of Chromatography. B, Biomedical Applications
R AzumaT Satoh

Abstract

It has been suggested that GTS-21 can improve the learning deficits and inhibit the neuro-degeneration in patients with Alzheimer's disease. This paper describes a reversed-phase high-performance liquid chromatographic assay with visible detection at 405 nm for determination of GTS-21 and its metabolite, 4-hydroxy-GTS-21 in rat plasma. The method uses solid-phase extraction with a Bond Elut C18 column. A quantitation limit of 1.0 ng/ml was achieved using 0.5 ml of rat plasma. In the validation study, the coefficients of variation and the relative errors of each compound were less than 10%. Also freeze-thaw and storage stability were confirmed. This method has proved to be applicable to the pharmacokinetic study of GTS-21 in rats.

References

Sep 1, 1985·Environmental Science & Technology·T C Voice, W J Weber

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Citations

Aug 14, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·R AzumaH Kiwada
Mar 27, 2002·Biological & Pharmaceutical Bulletin·Hideki Kawamata, Kakuji Tojo
Mar 12, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Harumi KitagawaAngela L Burnett
Oct 1, 2015·Pharmacological Reviews·Daniel BertrandDiana Donnelly-Roberts
Aug 24, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R AzumaJ M Mathews

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