Simultaneous determination of hydrochlorothiazide, quinapril and quinaprilat in human plasma by liquid chromatography-tandem mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Sagar A ParekhSudhir S Kamat

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the simultaneous estimation of hydrochlorothiazide, quinapril and its metabolite quinaprilat in human plasma. After solid phase extraction (SPE), the analytes and IS were chromatographed on a hypurity C8 (100 mm x 2.1 mm i.d., 5 microm particle size) column using 2 microL injection volume with a run time of 2.8 min. An isocratic mobile phase consisting of 0.5% (v/v) formic acid:acetonitrile (25:75, v/v) was used to separate all these drugs. The precursor and product ions of these drugs were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring mode (MRM) without polarity switch. The proposed method was validated over the range of 5-500 ng/mL for hydrochlorothiazide method and 5-1500 ng/mL for quinapril and quinaprilat. Inter-batch and intra-batch precision (coefficient of variation - % CV) across five validation runs lower limit of quantitation (LLOQ), lower quality control (LQC), middle quality control (MQC), higher quality control (HQC) and upper limit of quantitation (ULOQ) was less than 15. The accuracy determined at these levels was within +/-13% in terms of...Continue Reading

References

Jul 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·R B Miller, C Amestoy
Jan 10, 1986·Journal of Chromatography·K B AltonJ E Patrick
May 11, 1984·Journal of Chromatography·P P KoopmansF W Gribnau
Feb 5, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ch AbbaraB Diquet
Jun 3, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takatoshi TakuboYasuyuki Ishii
Jun 15, 2007·Journal of Pharmaceutical and Biomedical Analysis·Fei LiuQingxiang Guo

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