Simultaneous determination of hydrolysis and mutarotation rates during the enzymatic hydrolysis of lactose

Journal of Agricultural and Food Chemistry
Daniel M JenkinsOwen Young

Abstract

An experiment is described in which a custom-made glucose electrode is used to directly monitor the enzymatic hydrolysis of lactose to glucose. The transient profile of beta- d-glucose can be used to simultaneously determine the rate constants for mutarotation and for enzymatic hydrolysis by applying a dynamic nonlinear regression routine. Due to differences in the mutarotation rate constants between lactose and glucose, the beta- d-glucose concentration "overshoots" equilibrium under certain conditions, which can be modeled mathematically. This overshoot can be observed reliably and used to quantify the differences in mutarotational equilibria between glucose and lactose. These observations may be important for the analysis of dairy products and commercial lactase preparations and illustrate an unusual kinetic phenomenon caused by intramolecular forces. This approach may also be important for the accurate determination of a variety of oligosaccharides such as glycogen, which tend to be composed primarily of one stereoisomer.

References

Oct 1, 1976·Analytical Chemistry·F R Shu, G S Wilson
Feb 1, 1966·Journal of Dairy Science·G Haase, T A Nickerson
Apr 1, 1984·Analytical Chemistry·A E CassA P Turner
Nov 26, 2002·Biosensors & Bioelectronics·Daniel M Jenkins, Michael J Delwiche
Oct 31, 1962·Annals of the New York Academy of Sciences·L C CLARK, C LYONS
Apr 6, 2006·Carbohydrate Research·Alexander M SilvaClarissa O da Silva

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