Simultaneous determination of key bioactive components in Hedyotis diffusa by capillary electrophoresis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
H Y CheungW P Lai

Abstract

A capillary zone electrophoresis (CZE) method based on systematic one-variable-at-a time approach was developed for the analysis of four important bioactive components (geniposidic acid, ursolic acid, quercetin and p-coumaric acid) in the extract of Hedyotis diffusa (HD). Separations were carried out in a fused-silica capillary tube with peak detection at 214 nm. Good separation was achieved using a 20 mM borate buffer containing 5% acetonitrile as organic modifier and pH adjusted to 10.0. Operating voltage was 15 kV and temperature was maintained at 25 degrees C while hydrodynamic injection was 5s. A good linearity, with correlation coefficients in the ranges of 0.997-0.999 was obtained in the calibration curves of each standard. Relative standard deviation (R.S.D.) of migration time was between 0.32 and 0.70% and deviation of corrected peak area was between 8.84 and 11.99%. These results indicate that this method could be used for rapid and simultaneous analysis of the bioactive components in HD and other herbal products.

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Citations

Apr 23, 2013·Journal of Asian Natural Products Research·Yu Niu, Qiu-Xia Meng
Sep 7, 2007·Electrophoresis·Christian W Klampfl
Sep 9, 2016·Journal of Zhejiang University. Science. B·Jian-Zhen ShanJian-Jin Huang
Jun 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui ChenMenghua Liu
May 18, 2021·Frontiers in Plant Science·Amanda FanelliElisson Romanel

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