Simultaneous determination of lamotrigine and its glucuronide and methylated metabolites in human plasma by automated sequential trace enrichment of dialysates and gradient high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
J D CooperC T Fook Sheung

Abstract

The use of the system, automated sequential trace enrichment of dialysates (ASTED), to prepare plasma samples for the estimation of lamotrigine, its glucuronide and methylated metabolites in plasma prior to gradient high-performance liquid chromatography (HPLC) is described. Using this technique the procedure was observed to be specific for all three compounds and linear over the range 0.04 to 10 microg/ml for lamotrigine and the glucuronide metabolite and 2 to 500 ng/ml for the methylated metabolite. The within-run precision (C.V.) at four different supplemented plasma lamotrigine concentrations of 0.04, 0.10, 2.5 and 10.0 microg/ml was 6.21, 5.17, 1.29 and 0.73%, respectively, and the between-run precision (C.V.) estimated to be 13.49, 6.08, 1.95 and 1.78%, respectively. The overall accuracy (% bias) of the procedure was estimated to be 12.50, 0.00, 2.80 and 1.80%, respectively. The glucuronide and methylated metabolites in plasma showed similar assay performance.

References

Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·R A BiddlecombeS C Jeal

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Citations

Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel F Chollet
Jun 26, 2001·British Journal of Clinical Pharmacology·P MarcellinR Bidault
Dec 6, 2011·Bioelectrochemistry·Reyhaneh-Sadat Saberi, Saeed Shahrokhian
May 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maria Shipkova, Eberhard Wieland
Aug 9, 2007·Journal of Separation Science·Maria Addolorata SaracinoMaria Augusta Raggi

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