Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Hoang Anh NguyenM C Saux

Abstract

Liquid chromatography with a column-switching technique was developed for simultaneous direct quantification of levofloxacin, gatifloxacin and moxifloxacin in human serum. Serum samples were injected on a LiChroCART 4-4 pre-column (PC) filled with a LiChrospher 100 RP-18, 5 microm where fluoroquinolones (FQs) were purified and concentrated. The FQs were back-flushed from the PC and then separated on a Supelcosil ABZ+ Plus (150 mm x 4.6 mm i.d.) analytical column with a mobile phase containing 10 mM phosphate buffer (pH 2.5), acetonitrile (88:12, v/v) and 2mM tetrabutyl ammonium bromide. The effects of ion-pair reagents, buffer type, pH and acetonitrile concentrations in the mobile phase on the separation of the three FQs were investigated. Fluorescence detection provided sufficient sensitivity to achieve a quantification limit of 125 ng/ml for levofloxacin and moxifloxacin; 162.5 ng/ml for gatifloxacin with a 5 microl sample size. The on-line process of extraction avoids time-consuming treatment of the samples before injection and run time is shortened. The recovery, selectivity, linearity, precision and accuracy of the method are convenient for pharmacokinetic studies or routine assays.

References

Mar 1, 1995·Antimicrobial Agents and Chemotherapy·K V Clemons, D A Stevens
Sep 22, 1993·Journal of Chromatography·P Campíns-FalcóA Sevillano-Cabeza
Dec 1, 1995·Antimicrobial Agents and Chemotherapy·M NakashimaH Uchida
Feb 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·S BraggioM Cugola
Feb 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·K Fried, I W Wainer
Nov 14, 1997·Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography·M L MainP A Grayburn
Sep 17, 1998·The Journal of Antimicrobial Chemotherapy·C M TobinD S Reeves
May 1, 1999·The Journal of Antimicrobial Chemotherapy·C M TobinA P MacGowan
Apr 25, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·B B BaM C Saux
May 1, 2001·Clinical Pharmacokinetics·A AminimanizaniR Jelliffe
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hairui LiangKevin M Sowinski
Oct 31, 2008·Talanta·F BelalA M Al-Obaid

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Citations

Jan 8, 2008·Chemical & Pharmaceutical Bulletin·Ayman Abouel Fetouh GoudaAlaa Sayed Amin
Feb 17, 2015·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Ibrahim A AljuffaliAws Alshamsan
Oct 21, 2009·Journal of Separation Science·Zhifeng FuWei Wei
Jan 18, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Safwan Ashour, Roula Bayram

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