PMID: 12576054Feb 11, 2003

Simultaneous determination of methionine sulfoxide and methionine in blood plasma using gas chromatography-mass spectrometry

Analytical Biochemistry
Ryuichi MashimaYorihiro Yamamoto

Abstract

Methionine sulfoxide is an oxidation product of methionine with reactive oxygen species via 2-electron-dependent mechanism. Such oxidants can be generated from activated neutrophils; therefore, methionine sulfoxide can be regarded as a biomarker of oxidative stress in vivo. We describe here a method for the simultaneous determination of methionine sulfoxide and methionine in blood plasma using gas chromatography-mass spectrometry with isotopically labeled compounds as internal standards. This method comprises the inclusion of [Me-13C, Me-2H(3)]methionine sulfoxide and [Me-13C, Me-2H(3)]methionine into plasma, the removal of plasma proteins using acetonitrile, the purification of amino acids with cation-exchange chromatography, and the derivatization of methionine sulfoxide and methionine to their corresponding tert-butyldimethylsilyl derivatives using N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide. Quantitation was performed by electron impact mode. The levels of methionine sulfoxide in healthy human blood plasma were 4.0 +/- 1.0 microM (means +/- SD, n = 8), indicating that approximately 10% of methionine is detected as the oxidized form in healthy human plasma. The ratio of methionine sulfoxide in total methionine inc...Continue Reading

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Citations

Jun 7, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Oemer-Necmi GoekChrista Meisinger
Aug 9, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Joseph T Dever, Adnan A Elfarra
Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Matthew D HallMichael M Gottesman
Nov 14, 2013·BMC Physiology·Alexander G TonevitskyHinnak Northoff
Apr 23, 2005·Biochemical and Biophysical Research Communications·Haruka FukamachiYoshihisa Yamashita
Sep 30, 2010·Expert Opinion on Drug Metabolism & Toxicology·Joseph T Dever, Adnan A Elfarra
Apr 8, 2016·PloS One·Jung-Eun LeeYoungseok Lee
Feb 9, 2017·Journal of Proteome Research·Wiebke Maria NadlerChristoph Rösli
Mar 7, 2017·Nature Reviews. Nephrology·Berthold Hocher, Jerzy Adamski
Feb 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lucie AumailleyMichel Lebel
Aug 5, 2003·Journal of Mass Spectrometry : JMS
Apr 23, 2015·Clinical Journal of the American Society of Nephrology : CJASN·Kristen L JablonskiDouglas R Seals
Nov 28, 2019·Metabolomics : Official Journal of the Metabolomic Society·Katrin N SanderDavid A Barrett
Sep 8, 2017·Movement Disorders : Official Journal of the Movement Disorder Society·Wei HanLiang Li
May 26, 2020·Nanotoxicology·Anne BannuscherAndrea Haase
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Jan 16, 2021·Free Radical Biology & Medicine·Ting GuiZhibo Gai
Mar 4, 2021·Translational Psychiatry·Siamak MahmoudianDehkordiMood Disorders Precision Medicine Consortium (MDPMC)
Apr 7, 2021·Molecular Systems Biology·Simon A CobboldMalcolm J McConville

Related Concepts

Methionine sulfoxide,35S-labeled, (+-)-isomer
Carbon Isotopes
Deuterons
Chromatography, Gas-Liquid-Mass Spectrometry
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