Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection

Biomedical Chromatography : BMC
Steffen BauerIvar Roots

Abstract

A rapid and sensitive HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5-HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liquid-liquid extraction of human plasma with dichloromethane coupled with reversed-phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra- and inter-assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters.

References

Sep 1, 1991·Journal of Pharmaceutical Sciences·P V ColthupN L Scully
Jun 6, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·M DépôtG Caillé
Oct 14, 2000·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·P S LoewenP J Zed

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Citations

Jul 1, 2005·Environmental Toxicology and Pharmacology·Seung Wei JeongJong-Koo Kang
Oct 14, 2008·International Journal of Pharmaceutics·Yara Popst ArmandoCristina Helena dos Reis Serra
Aug 13, 2008·Biopharmaceutics & Drug Disposition·Si H YangMyung G Lee
Sep 12, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Li YuXingjie Guo
Apr 7, 2020·Drug Development and Industrial Pharmacy·Mahmoud H TeaimaAmir I Mohamed

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