Simultaneous determination of polyamines and acetylpolyamines in human urine by capillary electrophoresis with fluorescence detection

Electrophoresis
Abdalla A ElbashirOliver J Schmitz

Abstract

There has been evidence linking elevated polyamines (PAs) and acetylpolamines (AcPAs) level and cancer. So the simultaneous analysis of these compounds has become important task for cancer diagnosis and antitumor drug monitoring. A simple, fast and inexpensive CZE-LIF method has been developed for the determination of cadaverine (CAD), putrescine (PUT), spermine (SPM), spermidine (SPD), acetylspermine (ASPM), and acetylspermidine (ASPD) in human urine using 4-chloro-7-nitro-2,1,3-benzooxadiazole as a fluorescent reagent. Labeling reaction conditions were systematically investigated and were found to be 20 mM borate buffer at pH 7.4, labeling reaction time, and temperature were 10 min and 70°C, respectively. Under these optimized conditions the four PAs, two AcPAs and the internal standard were separated in 6 min. An Exactive-MS with an ESI source was used for identification of the bis-derivative of the ASPM. The method was validated in term of linearity, LODs, repeatability, intra- and interday assays, recovery, and selectivity. The LODs for CAD, PUT, SPM, SPD, ASPM, and ASPD were found to be 7.6, 10.0, 9.0, 8.8,7.8, and 3.3 nM, respectively. The method was successfully applied for the analysis of PAs and AcPAs in healthy human...Continue Reading

References

Mar 13, 1981·Journal of Chromatography·M M Abdel-Monem, J L Merdink
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·L J Marton, A E Pegg
Jan 24, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·J W SuhJ Park
Oct 24, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·G TaibiC M Nicotra
Feb 24, 2001·Electrophoresis·J C WatervalW J Underberg
Feb 24, 2001·Journal of Chromatography. a·B X Mayer
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M VenzaD Teti
Aug 17, 2001·Biomedical Chromatography : BMC·S UchiyamaK Imai
Dec 14, 2002·Electrophoresis·Willy J M Underberg, Joop C M Waterval
Mar 24, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Christophe MoinardJean-Pascal de Bandt
May 5, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Li-Yao ZhangMeng-Xiang Sun
Nov 7, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Valentin LozanovVanio Mitev
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Eva Szöko, Tamás Tábi

❮ Previous
Next ❯

Citations

Sep 5, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alan Alexander González IbarraKazimierz Wrobel
Sep 13, 2015·Journal of Chromatography. a·Daniela DanielClaudimir Lucio do Lago
Aug 25, 2020·Critical Reviews in Analytical Chemistry·Abdalla Ahmed ElbashirHassan Y Aboul-Enein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.