PMID: 7134801Apr 1, 1982Paper

Simultaneous determination of prednisolone and cortisol in serum by HPLC and by isotope dilution--mass spectrometry

Scandinavian Journal of Clinical and Laboratory Investigation
L OstI Björkhem

Abstract

A reference method for the simultaneous assay of cortisol and prednisolone by isotope dilution-mass spectrometry has been developed. A fixed amount of 2H4-labelled cortisol is added to a fixed amount of serum. The mixture is then extracted, converted into methoxime-trimethylsilyl derivative, and subjected to analysis by combined gas chromatography-mass spectrometry. The ions at m/e 603, m/e 605 and m/e 609 are followed through the gas chromatography. These ions correspond to the M-31 ions in the mass spectrum of methoxime-trimethylsilyl ether derivative of prednisolone, unlabelled cortisol and 2H4-cortisol, respectively. With the use of a standard curve, the concentration of prednisolone can be calculated from the ratio between the peak at m/e 603 and the peak at m/e 609 and the concentration of cortisol can be calculated from the ratio between the peak at m/e 605 and the peak at m/e 609. The coefficient of variation was about 2% with respect to determination of prednisolone and about 3% with respect to determination of cortisol. This method was compared with a routine method based on high-performance liquid chromatography (HPLC) The coefficient of variation was 2-3% with respect to determination of cortisol and 1-3% with respe...Continue Reading

Citations

Jan 1, 1984·European Journal of Clinical Pharmacology·L OstC von Bahr
Jun 1, 1985·The Journal of Pediatrics·L OstA Rane

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