Simultaneous determination of propranolol and 4-hydroxypropranolol in plasma by mass fragmentography

Journal of Chromatography
T WalleE C Conradi

Abstract

A quantitative method for the simultaneous determination of propranolol and its active metabolite 4-hydroxypropranolol in human plasma is described. Plasma samples are extracted at pH 9.6 with ethyl acetate after the addition of sodium bisulphite and the internal standard oxprenolol. The extracts are derivatized with trifluoroacetic anhydride before separation on a gas chromatograph--mass spectrometer. Detection and quantitation of the trifluoroacetyl derivatives are made by single-ion monitoring. The minimum detectable concentration of propranolol is 1 ng/ml and of 4-hydroxypropranolol 5 ng/ml using 1-ml plasma samples. No interferences from normal plasma constituents or from drugs commonly prescribed together with propranolol were detected.

Citations

Sep 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·P S RaoH S Mueller
Dec 1, 1978·European Journal of Pharmacology·S R NahorskiD B Barnett
Jul 15, 1994·Journal of Chromatography. a·P LukkariM L Riekkola
May 10, 1985·Journal of Chromatography·M AhnoffJ Vessman
Mar 1, 1979·Journal of Chromatography·D E EasterlingT E Gaffney
Aug 1, 1979·British Journal of Clinical Pharmacology·L WongP K Mehta
Apr 1, 1991·Journal of Clinical Pharmacy and Therapeutics·A A al-AngaryG M Mahrous
Jan 1, 1983·The American Journal of Cardiology·D H IansmithJ P Bandura
May 1, 1989·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B C FosterI J McGilveray
Mar 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T WalleT E Gaffney
Sep 1, 1986·British Journal of Clinical Pharmacology·T WalleE C Conradi
Aug 1, 1983·Analytical Chemistry·T D EllerT Walle
Sep 1, 1978·Circulation·P Hanrath, D Mathey
Jun 1, 1985·Journal of Pharmacokinetics and Biopharmaceutics·S A BaiT Walle
May 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G W MihalyK J Hardy

Related Concepts

Chromatography, Gas-Liquid
Hydrogen-Ion Concentration
Chromatography, Gas-Liquid-Mass Spectrometry
Study, Methodological
Rexigen

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.