Simultaneous determination of pyrimethamine, sulfadiazine and N-acetyl-sulfadiazine in plasma for monitoring infants in treatment of congenital toxoplasmosis

Journal of Pharmaceutical and Biomedical Analysis
Jane Krogh JohannessenSteen Honoré Hansen

Abstract

A method for the simultaneous determination of pyrimethamine, sulfadiazine and its metabolite N-acetyl-sulfadiazine in small plasma samples from neonates in treatment for congenital toxoplasmosis has been developed. In this method only 25 microl of plasma is used and a simple sample preparation based on protein precipitation and centrifugation provides highly reliable data as the recovery is about 100% and the precision is good. The analysis is performed using high performance liquid chromatography with UV and mass spectrometric (MS) detection. Pyrimethamine was found to give a linear response using MS detection in the range 0.02-5 microg/ml. Sulfadiazine and its metabolite N-acetyl-sulfadiazine were preferably analysed by UV at 269 nm in the concentration ranges 0.2-200 microg/ml for sulfadiazine and 0.2-50 microg/ml for N-acetyl-sulfadiazine.

Citations

Jun 30, 2010·Journal of Inherited Metabolic Disease·Dennis RöserPeter Bent Nørgaard-Pedersen
Nov 21, 2009·La Presse médicale·Patricia Garcia-MéricRenaud Piarroux
Dec 10, 2015·Journal of Pharmacological and Toxicological Methods·Lisa Amanda Holm Rottbøll, Christian Friis
Mar 20, 2008·Survey of Ophthalmology·Marilyn Mets, Manpreet Singh Chhabra
Jun 10, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Monica Escolà CasasErland Björklund
Jun 14, 2014·Andrology·O Gutiérrez-PérezR M Vigueras-Villaseñor
Feb 24, 2021·Journal of Chromatographic Science·Hina ShamshadAgha Zeeshan Mirza

Related Concepts

High Pressure Liquid Chromatography Procedure
Malocide
Spectrophotometry, Ultraviolet
Mass Spectrometry
Sulfazin
Toxoplasmosis, Fetal
Therapeutic Drug Monitoring

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