Abstract
Eicosanoids are biologically active lipid-derived oxidative metabolites of arachidonic acid. We, herein, present an improved sensitive, selective and robust high performance liquid chromatography (HPLC)-fluorescence assay for simultaneous quantification of eicosanoids in human plasma and rat tissues. Aliquots of 200 μL of plasma or 30 mg of heart or kidney tissues were spiked with 16-hydroxydecanoic acid as internal standard, and extracted with anhydrous acetonitrile using solid phase cartridges. The eluted samples were dried, reconstituted in anhydrous acetonitrile and labeled with 2-(2,3-naphthalimino)ethyl-trifluoromethanesulphonate in the presence of saturated potassium fluoride solution in anhydrous acetonitrile and N,N-diiospropylethylamine as catalyst. The derivatized eicosanoids were extracted with anhydrous acetonitrile using solid phase cartridges. Chromatographic separation was achieved on a C18 reversed phase column using gradient mobile phase of 0.05% of formic acid:acetonitrile:water at 0.8 mL/min flow rate. The analytes were detected at excitation and emission wavelength of 260 and 396 nm, respectively. The assay was linear (r(2)≥ 0.98) in the concentration range of 0.01-2.5 μg/mL. The intra-day and inter-day coe...Continue Reading
References
Feb 14, 1992·Biochemical and Biophysical Research Communications·A KararaJ R Falck
Mar 1, 1996·Circulation Research·W B CampbellD R Harder
May 1, 1997·Journal of Vascular Research·D R HarderR J Roman
Oct 31, 1998·Circulation Research·C L OltmanK C Dellsperger
Sep 19, 2000·Thorax·M A Carroll, J C McGiff
Nov 10, 2001·Analytical Biochemistry·K NithipatikomW B Campbell
Jan 5, 2002·Physiological Reviews·Richard J Roman
Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christopher A BolcatoSamuel M Poloyac
Feb 12, 2004·Biomedical Chromatography : BMC·Leonardo A MoraesDavid Perrett
Apr 6, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hongfei YueSusan A Jansen
May 25, 2005·Journal of Neuroscience Methods·Samuel M PoloyacMary E Kerr
Oct 10, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Ines UnterwurzacherSteven L Ramsay
Nov 7, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tricia M MillerSamuel M Poloyac
Nov 19, 2009·Archives of Biochemistry and Biophysics·T NilssonE H Oliw
Jan 5, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anwar Anwar-mohamedAyman O S El-Kadi
Oct 16, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Katherine N ThekenCraig R Lee
Sep 2, 2011·Journal of Pharmaceutical and Biomedical Analysis·Raquel Martín-VenegasJuan José Moreno
Dec 28, 2011·Biochimica Et Biophysica Acta·Victoria J Hammond, Valerie B O'Donnell
Mar 22, 2012·Medicinal Research Reviews·Valérie CapraG Enrico Rovati
Dec 12, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dhananjay D ShindeJae Gook Shin
May 11, 2013·Pulmonary Circulation·Kristen D SaglianiIoana R Preston
Jun 26, 2013·Journal of Pharmaceutical and Biomedical Analysis·Peter HoffmanPeter Mikuš
Oct 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vindhya Edpuganti, Reza Mehvar
Citations
Sep 24, 2015·Bioanalysis·Stacy Ho, Hong Gao
Nov 25, 2017·Bioanalysis·Mariana Millan FachiRoberto Pontarolo
Oct 5, 2017·Biomedical Chromatography : BMC·Rhishikesh ThakareYazen Alnouti
Apr 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika
Jul 31, 2019·Inflammopharmacology·Surur Ali AhmedFakhreddin Jamali
Jan 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika
Feb 6, 2020·Molecular Pharmaceutics·Hanan Al-LawatiFakhreddin Jamali
Sep 4, 2021·Analytical and Bioanalytical Chemistry·Kodjo NouwadePierre Chaminade