Simultaneous determination of selective serotonin reuptake inhibitors and their main metabolites in human breast milk by liquid chromatography-electrospray mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Etienne Weisskopfcollaborators of the SSRI-Breast Milk study

Abstract

A bioanalytical method by high performance liquid chromatography coupled to electrospray mass spectrometry (HPLC-ESI-MS), adapted from a previously published method in plasma, was validated in breast milk for the simultaneous quantification of all antidepressants belonging to the class of selective serotonin reuptake inhibitors (citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline) and their major metabolites (desmethylcitalopram and norfluoxetine). Milk samples (250μl) first underwent protein precipitation followed by solid-phase extraction on a reversed phase/cation exchange sorbent. Analytes were thereafter separated on a XBridge C18 column (2.1mm×100mm; 3.5μm) using a mobile phase composed of ammonium acetate buffer (pH 8.1; 50mM) and acetonitrile in gradient mode. Detection was performed by a single quadrupole mass spectrometer running in selected ion monitoring in positive ionization mode. Method validation covered a wide concentration range of 2-500ng/ml for citalopram, desmethylcitalopram and paroxetine, 5-500ng/ml for sertraline, and 2-1000ng/ml for fluoxetine, norfluoxetine and fluvoxamine. Validation performances such as trueness (90.3-111.6%), repeatability (0.8-9.3%) and intermediate precision (0.9-9.5%) ...Continue Reading

References

Sep 15, 1999·The Journal of Clinical Psychiatry·R OhmanO Spigset
Nov 27, 2002·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Evan J BeggKenneth F Ilett
Feb 20, 2003·The Journal of Clinical Psychiatry·Zachary N StoweCharles B Nemeroff
Aug 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·B BoulangerPh Hubert
Jan 30, 2004·Therapeutic Drug Monitoring·Amy L HostetterJames C Ritchie
Jun 1, 2004·The American Journal of Psychiatry·Alicia M WeissmanKatherine L Wisner
Apr 11, 2007·Journal of Chromatography. a·Eric RozetPhilippe Hubert
Oct 1, 2004·Paediatrics & Child Health
Jan 31, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nicolas AnsermotChin B Eap
Aug 28, 2013·Pediatrics·Hari Cheryl Sachs, UNKNOWN Committee On Drugs
Aug 9, 2016·Journal of Pharmaceutical and Biomedical Analysis·Fernanda Rodrigues SalazarAna Maria Bergold

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Citations

Mar 21, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Laura PoglianiGianvincenzo Zuccotti
Dec 12, 2019·Expert Review of Proteomics·Flaminia BardanzelluAlessandra Coscia
Dec 19, 2019·Pharmacogenetics and Genomics·Rachel HuddartTeri E Klein
Sep 8, 2020·Bioanalysis·Wilson José Ramos Santos, Bruno Spinosa De Martinis
Dec 29, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Georgios SchoretsanitisOlav Spigset

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