Simultaneous determination of six active components in traditional herbal medicine 'Oyaksungisan' by HPLC-DAD.

Journal of Natural Medicines
Jin Bae WeonChoong Je Ma

Abstract

In this study, an effective high performance liquid chromatography-diode array detector (HPLC-DAD) method was established for simultaneous determination of six marker compounds, ephedrine hydrochloride, 6-gingerol, glycyrrhizin, hesperidin, imperatorin and ferulic acid, in a Korean traditional prescription, Oyaksungisan, which is used for hemiplegia, arthralgia and paralysis. The six marker compounds of Oyaksungisan were separated on a LUNA C18 column (S-5 μm, 4.6 mm I.D. × 250 mm) at a column temperature of 35°C. The gradient elution was composed of water with 0.1% trifluoroacetic acid and methanol. The detection UV wavelengths were set at 207, 250, 280 and 320 nm. Calibration curves for the six compounds showed good linear regressions (R (2) > 0.9999). The limits of detection and limits of quantification were within the ranges 0.003-0.01 and 0.01-0.04 μg/ml, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were within the ranges 0.10-1.82 and 0.04-1.59%, respectively. The results of the recovery test were 95.05-104.27% with a RSD value of 0.11-1.85%. In conclusion, the simultaneous determination method developed was useful in the quality evaluation of Oyaksungisan.

References

Jun 29, 2004·World Journal of Gastroenterology : WJG·Ai-Ping LuQing-Ping Lu
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pavel Drasar, Jitka Moravcova
Sep 28, 2005·Trends in Pharmacological Sciences·Wen-Yue Jiang
Mar 4, 2008·Journal of Pharmaceutical and Biomedical Analysis·Chao FengJin-Lan Ruan
Dec 29, 2010·Journal of Natural Medicines·Jin Bae WeonChoong Je Ma

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Citations

Apr 30, 2013·Journal of Natural Medicines·Narisu BaoTakashi Yamagishi
Jul 28, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Ewelina Kozioł, Krystyna Skalicka-Woźniak
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Sylwia MagieraAnna Lautenszleger

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