Simultaneous determination of six major ergot alkaloids and their epimers in cereals and foodstuffs by LC-MS-MS

Analytical and Bioanalytical Chemistry
Rudolf KrskaColin Crews

Abstract

This paper describes a new and rapid method for accurate quantification of the six ergot alkaloids, ergometrine, ergotamine, ergosine, ergocristine, ergocryptine, and ergocornine, by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The six ergot alkaloids studied have been defined by the European Food Safety Authority (EFSA) as among the most common and physiologically active ones. In addition, the method enables the quantification of the corresponding six epimers (ergo-inines) of these ergot alkaloids. This is of considerable importance in terms of the differences in toxicity of the isomeric forms. The method involves extraction under alkaline conditions using a mixture of acetonitrile and ammonium carbonate buffer followed by a rapid clean-up using dispersive solid-phase extraction with PSA (primary secondary amine) and a short chromatographic LC-run (21 min) with subsequent MS-MS detection. The method was developed and validated using ten different cereal and food samples. The major strength of the new method compared with previously published techniques is the simplicity of the clean-up procedure and the short analysis time. The limits of quantification were 0.17 to 2.78 μg kg(-1) depending on the analyte and matr...Continue Reading

References

Jan 1, 1979·CRC Critical Reviews in Food Science and Nutrition·K Lorenz
Nov 1, 1980·Journal of Agricultural and Food Chemistry·P M Scott, G A Lawrence
May 1, 1995·Food Additives and Contaminants·P M Scott
Jul 17, 1999·Multiple Sclerosis : Clinical and Laboratory Research·A BitschW Brück
Mar 26, 2003·Zentralblatt für Gynäkologie·J Schlumbohm
Jul 15, 2003·Clinical Microbiology Reviews·J W Bennett, M Klich
Oct 8, 2004·Journal of Mass Spectrometry : JMS·Andreas F LehnerThomas Tobin
Jul 16, 2005·Food Additives and Contaminants·Rudolf KrskaB Mizaikoff
Mar 21, 2006·Molecular Nutrition & Food Research·Gisela BürkAnita Richt
May 20, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·R Krska, C Crews
Dec 1, 2005·Mycotoxin Research·U LauberY Kiesswetter
Dec 1, 2006·Mycotoxin Research·C MüllerR Wittkowski
Sep 1, 2007·Mycotoxin Research·P M Scott
Jun 1, 2009·Food Additives & Contaminants. Part B, Surveillance·C CrewsR Krska

❮ Previous
Next ❯

Citations

Sep 13, 2012·Analytical and Bioanalytical Chemistry·Stefan MerkelIrene Nehls
Mar 27, 2010·Applied Microbiology and Biotechnology·Robert KöppenIrene Nehls
Feb 1, 2012·Mycotoxin Research·Stefan MerkelIrene Nehls
Oct 22, 2013·Journal of Agricultural and Food Chemistry·Robert KöppenMatthias Koch
Jun 1, 2009·Food Additives & Contaminants. Part B, Surveillance·C CrewsR Krska
Apr 1, 2009·International Journal of Molecular Sciences·Irena Kralj Cigić, Helena Prosen
Jun 6, 2015·Toxins·Colin Crews
Mar 5, 2016·Frontiers in Veterinary Science·Stephanie Coufal-MajewskiYuxi Wang
Aug 19, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Natalia Arroyo-ManzanaresSarah De Saeger
Jul 3, 2015·Journal of Agricultural and Food Chemistry·Sheryl A TittlemierTwylla McKendry
Mar 6, 2015·Frontiers in Chemistry·A Morrie CraigJennifer M Duringer
Mar 24, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Dritan TopiGabrijela Tavčar-Kalcher
Jun 24, 2010·Journal of Separation Science·Meri Kokkonen, Marika Jestoi
Oct 26, 2010·Rapid Communications in Mass Spectrometry : RCM·Milena ZachariasovaJana Hajslova
May 6, 2019·Foods·Francesca DebegnachBarbara De Santis
Sep 6, 2020·Regulatory Toxicology and Pharmacology : RTP·Anne B CarlsonJason M Roper
Aug 1, 2009·Journal of Agricultural and Food Chemistry·Darrin SmithJames Strickland
May 1, 2021·Toxins·Rossalin YonpiamAhmad Al-Dissi
May 1, 2021·Toxins·Laura Carbonell-RozasLaura Gámiz-Gracia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Rudolf Krska, Colin Crews
Journal of Separation Science
Meri Kokkonen, Marika Jestoi
Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
I D StormH C B Hansen
© 2022 Meta ULC. All rights reserved