Simultaneous determination of some active ingredients in anti-viral preparations of traditional Chinese medicine by micellar electrokinetic chromatography

Biomedical Chromatography : BMC
Xiaofeng ChenZhide Hu

Abstract

A simple and rapid micellar electrokinetic chromatography method was developed for the simultaneous determination of quercetin, gentiopicrin, forsythin, chlorogenic acid and caffeic acid in anti-viral preparations of traditional Chinese medicine (apTCM). In this method, the effects of buffer pH, concentration of the borax and SDS, organic modifiers, applied voltage and temperature on the separation were tested and discussed. The results showed that the fi ve analytes could be well separated within 11 min under conditions of 40 mM borax (pH 9.65) containing 20 mM SDS, 20 kV, at 25 degrees C. In the tested concentration range, regression equations revealed good linear relationships (correlation coefficients 0.9920-0.9991) between the peak areas and corresponding concentrations. In addition, a multiple linear regression QSPR model was constructed to predict the migration times of the analytes and the results were satisfactory. The method was validated by analysis of the five compounds in three representative apTCM samples with recoveries ranging from 89.2 to 106.6%.

References

Sep 1, 1989·Chemical & Pharmaceutical Bulletin·A I el-SedawyT Namba
Mar 15, 1997·Archives of Biochemistry and Biophysics·W J ArionA W Herling
Apr 1, 1997·Pharmaceutical Research·L A HollandS M Lunte
Mar 21, 1998·Bioscience, Biotechnology, and Biochemistry·Y KonoH Shibata
Jan 11, 2001·European Journal of Clinical Pharmacology·I ErlundA Aro
Apr 11, 2001·Journal of Chromatography. a·S J SheuW C Weng
Jan 5, 2002·Fresenius' Journal of Analytical Chemistry·U Pyell
Apr 11, 2003·Journal of Chromatography. a·Merike Vaher, Mihkel Koel

❮ Previous
Next ❯

Citations

Aug 4, 2009·European Journal of Drug Metabolism and Pharmacokinetics·Yun-Xia LiXue-Hua Jiang
Feb 22, 2011·European Journal of Drug Metabolism and Pharmacokinetics·Yun-Xia LiCheng Peng
Jun 1, 2013·Journal of Cheminformatics·Hong KangZhiwei Cao
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pavel Drasar, Jitka Moravcova
Nov 22, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Xia WangLing Yang
Sep 7, 2007·Electrophoresis·Christian W Klampfl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.