PMID: 18724674Aug 30, 2008Paper

Simultaneous determination of sulfonamides and fluoroquinolones residues in chicken by high performance liquid chromatography-electrospray tandem mass spectrometry

Se pu = Chinese journal of chromatography
Pengyan LiuLizhi Yan

Abstract

A method was developed for the simultaneous determination of 10 residual antibiotics--three sulfonamides (sulfadiazine, sulfamethazine and sulfamethoxydiazine) and seven fluoroquinolones (enoxacin, norfloxacin, enrofloxacin, fleroxacin, levofloxacin, lomefloxacin and ciprofloxacin) in chicken by high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS2). The sample was extracted with 2% acetic acid-acetonitrile for three times and defatted with n-hexane, then purified by an ENVI-18 solid-phase extraction column. After evaporation, the residue was dissolved in the mobile phase of 1 mL. The HPLC separation was performed with a gradient elution program of acetonitrile and water (containing 0.05% formic acid) as the mobile phase. The identification and quantification were done with mass spectrometry. MS2 fragmentation pathways of the ten antibiotics were also analyzed. Good linearities were observed in the range from 0.02 to 2.0 mg/L with correlation coefficients above 0.998 8. The limits of detection (LOD) were 1.10 -6.85 microg/kg and the limits of quantification (LOQ) were 3.68 -22.85 microg/kg. The average recoveries of 10 antibiotics ranged from 68.9% to 102.6% with relative standard deviations...Continue Reading

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