Simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in blood serum by high-performance liquid chromatography with direct injection

Journal of Chromatography. B, Biomedical Applications
K Uno, I Maeda


A high-performance liquid chromatographic method with direct injection has been developed for the simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in serum of animals and fish. A HISEP shielded hydrophobic-phase column (15 cm x 4.6 mm I.D.), a mobile phase of 0.05 M citric acid-0.2 M disodium hydrogenphosphate-acetonitrile (70:15:15, v/v), and ultraviolet detection at 265 nm were used. The standard calibration curves in serum of chicken, pig, cattle, rainbow trout and yellowtail were linear over the range 0.5-20 micrograms/ml. The recoveries of sulphamonomethoxine and its N4-acetyl metabolite from all serum samples determined at different concentrations (0.5, 2.0 and 10.0 micrograms/ml) were 93-103% and 90-103%, respectively. The lowest measurable sulphamonomethoxine and N4-acetyl metabolite concentrations were 0.04 and 0.1 microgram/ml, respectively, for all serum samples.


Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S SouverainJ-L Veuthey
Dec 22, 2012·Se pu = Chinese journal of chromatography·Xiaoyu WuXianhua Wang

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